O6-Methyl-2′-deoxyguanosine (O6-Me-dG) is a mutagenic nucleotide in DNA. O6-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O6-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O6-Me-dG in DNA.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry