An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon

Naoto Shirahata, Atsushi Hozumi, Yoshiko Miura, Kazukiyo Kobayashi, Yoshio Sakka, Tetsu Yonezawa

研究成果: ジャーナルへの寄稿Conference article

9 引用 (Scopus)

抜粋

Efficient matrix that resists the nonspecific adsorption of proteins from the contacting phosphate buffer silane (PBS) solution has been explored to fabricate carbohydrate arrays on silicon wafer. p-Vinylbenzyllactonoamide (VLA) was immobilized onto silicon through Si-C bond. The VLA-covered substrate was then photolithographically micropatterned through a photomask by ultraviolet (UV) irradiation. The VLA-covered regions recognized specifically the lactose-binding lectin in PBS solution. On the other hand, the UV-irradiated regions, i.e., SiOx surface, prevented the nonspecific adsorption of the lectin from the contacting PBS solution. Several control experiments have demonstrated that the resistant-ability of SiOx surface as the matrix was comparable to the best of known system for resisting the nonspecific adsorption.

元の言語英語
ページ(範囲)213-218
ページ数6
ジャーナルThin Solid Films
499
発行部数1-2
DOI
出版物ステータス出版済み - 3 21 2006
外部発表Yes
イベントProceedings of the Sixth International Conference on Nano-Molecular Electronics (ICNME 2004) -
継続期間: 12 15 200412 17 2004

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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