An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I

Watoo Phrompittayarat, Waraporn Putalun, Hiroyuki Tanaka, Sakchai Wittaya-Areekul, Kanchalee Jetiyanon, Kornkanok Ingkaninan

研究成果: ジャーナルへの寄稿学術誌査読

24 被引用数 (Scopus)


Bacopa monnieri (L.) Wettst. (Brahmi) is a medicinal plant used as a memory enhancer in Ayurvedic medicines. Its active components are triterpenoid glycosides namely pseudojujubogenin and jujubogenin glycosides. In order to analyze these saponin glycosides, an enzyme-linked immunosorbant assay (ELISA) was developed using polyclonal antibodies against bacopaside I, one of the pseudojujubogenin glycosides found in the plant. Bacopaside I was conjugated with a bovine albumin serum (BSA) to prepare an immunogen. The bacopaside I-BSA conjugate was immunized to a rabbit for producing polyclonal antibodies (PAbs). The results showed that the antibodies were raised specifically against pseudojujubogenin glycosides. An ELISA using anti-bacopaside I PAbs was performed in the range of 1.95-62.5 ng mL-1 of bacopaside I and the limit of detection was 0.1 ng mL-1. The method was validated and the applicability of the ELISA for analyzing saponin glycosides from Brahmi was demonstrated.

ジャーナルAnalytica Chimica Acta
出版ステータス出版済み - 2月 12 2007

!!!All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生化学
  • 環境化学
  • 分光学


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