TY - JOUR
T1 - An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I
AU - Phrompittayarat, Watoo
AU - Putalun, Waraporn
AU - Tanaka, Hiroyuki
AU - Wittaya-Areekul, Sakchai
AU - Jetiyanon, Kanchalee
AU - Ingkaninan, Kornkanok
N1 - Funding Information:
The grant from National Research Council of Thailand is gratefully acknowledged. The authors thank Naresuan University for a Ph.D. scholarship of the first author. Special thanks for Assoc. Prof. Dr. Wongsatit Chawkul for the identification of Brahmi. Professor I. Khan was acknowledged for giving saponin standard references for this study.
PY - 2007/2/12
Y1 - 2007/2/12
N2 - Bacopa monnieri (L.) Wettst. (Brahmi) is a medicinal plant used as a memory enhancer in Ayurvedic medicines. Its active components are triterpenoid glycosides namely pseudojujubogenin and jujubogenin glycosides. In order to analyze these saponin glycosides, an enzyme-linked immunosorbant assay (ELISA) was developed using polyclonal antibodies against bacopaside I, one of the pseudojujubogenin glycosides found in the plant. Bacopaside I was conjugated with a bovine albumin serum (BSA) to prepare an immunogen. The bacopaside I-BSA conjugate was immunized to a rabbit for producing polyclonal antibodies (PAbs). The results showed that the antibodies were raised specifically against pseudojujubogenin glycosides. An ELISA using anti-bacopaside I PAbs was performed in the range of 1.95-62.5 ng mL-1 of bacopaside I and the limit of detection was 0.1 ng mL-1. The method was validated and the applicability of the ELISA for analyzing saponin glycosides from Brahmi was demonstrated.
AB - Bacopa monnieri (L.) Wettst. (Brahmi) is a medicinal plant used as a memory enhancer in Ayurvedic medicines. Its active components are triterpenoid glycosides namely pseudojujubogenin and jujubogenin glycosides. In order to analyze these saponin glycosides, an enzyme-linked immunosorbant assay (ELISA) was developed using polyclonal antibodies against bacopaside I, one of the pseudojujubogenin glycosides found in the plant. Bacopaside I was conjugated with a bovine albumin serum (BSA) to prepare an immunogen. The bacopaside I-BSA conjugate was immunized to a rabbit for producing polyclonal antibodies (PAbs). The results showed that the antibodies were raised specifically against pseudojujubogenin glycosides. An ELISA using anti-bacopaside I PAbs was performed in the range of 1.95-62.5 ng mL-1 of bacopaside I and the limit of detection was 0.1 ng mL-1. The method was validated and the applicability of the ELISA for analyzing saponin glycosides from Brahmi was demonstrated.
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U2 - 10.1016/j.aca.2006.11.017
DO - 10.1016/j.aca.2006.11.017
M3 - Article
C2 - 17386577
AN - SCOPUS:33846266526
VL - 584
SP - 1
EP - 6
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1
ER -