An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans

Masahito Kawasaki, Nobumi Takamatsu, Toshihiro Ansai, Yoshihisa Yamashita, Tadamichi Takehara, Toshihiko Koga

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

An enzyme-linked immunosorbent assay utilizing avidin-biotin binding has been developed for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. The serotype a, b and c polysaccharide antigens were coupled with biotin via an adipic acid hydrazide functional group. The biotinylated antigens immobilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomycetemcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutions of human sera from subjects with periodontitis could be detected by using the assay.

元の言語英語
ページ(範囲)181-192
ページ数12
ジャーナルJournal of Microbiological Methods
21
発行部数2
DOI
出版物ステータス出版済み - 1 1 1995

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Aggregatibacter actinomycetemcomitans
Polysaccharides
Enzyme-Linked Immunosorbent Assay
Antigens
Avidin
Antibodies
Biotin
Periodontitis
Immune Sera
Rabbits
Serogroup
Serum

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Microbiology
  • Microbiology (medical)
  • Biotechnology
  • Applied Microbiology and Biotechnology

これを引用

An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. / Kawasaki, Masahito; Takamatsu, Nobumi; Ansai, Toshihiro; Yamashita, Yoshihisa; Takehara, Tadamichi; Koga, Toshihiko.

:: Journal of Microbiological Methods, 巻 21, 番号 2, 01.01.1995, p. 181-192.

研究成果: ジャーナルへの寄稿記事

Kawasaki, Masahito ; Takamatsu, Nobumi ; Ansai, Toshihiro ; Yamashita, Yoshihisa ; Takehara, Tadamichi ; Koga, Toshihiko. / An enzyme-linked immunosorbent assay for measuring antibodies to serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans. :: Journal of Microbiological Methods. 1995 ; 巻 21, 番号 2. pp. 181-192.
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