An estimated quantitative lateral flow immunoassay for determination of artesunate using monoclonal antibody

Tharita Kitisripanya, Witcha Sermpradit, Seiichi Sakamoto, Hiroyuki Tanaka, Waraporn Putalun

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

There have been reports of fake artesunate (ART), which has led to deaths from untreated malaria in South East Asia. To rapidly screen for fake and adulterated ART products in the drug market, a lateral flow immunoassay (LFIA) based on a colloidal gold–monoclonal antibody probe for detection of ART within samples was developed. With this method, the calibration curve for ART was determined by the intensity ratio of the test and control bands at various ART concentrations. The linearity range was 12.5–200 μg/ml of ART. Samples were tested by the developed LFIA and can be calculated for ART contents. The levels of ART in the samples were also confirmed by enzyme-linked immunosorbent assay. The results of the two methods were in good conformance. The proposed LFIA was demonstrated to be a simple and rapid analytical method for detecting ART in the pharmaceutical formulation.

本文言語英語
論文番号e4718
ジャーナルBiomedical Chromatography
34
2
DOI
出版ステータス出版済み - 2 1 2020

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生化学
  • 分子生物学
  • 薬理学
  • 創薬
  • 臨床生化学

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