An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of β-catenin

Masatoshi Kitagawa, Shigetsugu Hatakeyama, Michiko Shirane, Masaki Matsumoto, Noriko Ishida, Kimihiko Hattori, Ikuo Nakamichi, Akira Kikuchi, Kei Ichi Nakayama, Keiko Nakayama

研究成果: ジャーナルへの寄稿記事

445 引用 (Scopus)

抄録

β-catenin plays an essential role in the Wingless/Wnt signaling cascade and is a component of the cadherin cell adhesion complex. Deregulation of β-catenin accumulation as a result of mutations in adenomatous polyposis coli (APC) tumor suppressor protein is believed to initiate colorectal neoplasia. β-catenin levels are regulated by the ubiquitin-dependent proteolysis system and β-catenin ubiquitination is preceded by phosphorylation of its N-terminal region by the glycogen synthase kinase-3β (GSK-3β)/Axin kinase complex. Here we show that FWD1 (the mouse homologue of Slimb/βTrCP), an F-box/WD40-repeat protein, specifically formed a multi-molecular complex with β-catenin, Axin, GSK-3β and APC. Mutations at the signal-induced phosphorylation site of β-catenin inhibited its association with FWD1, FWD1 facilitated ubiquitination and promoted degradation of β-catenin, resulting in reduced cytoplasmic β-catenin levels. In contrast, a dominant-negative mutant form of FWD1 inhibited the ubiquitination process and stabilized β-catenin. These results suggest that the Skp1/Cullin/F-box protein FWD1 (SCF(FWD1))-ubiquitin ligase complex is involved in β-catenin ubiquitination and that FWD1 serves as an intracellular receptor for phosphorylated β-catenin. FWD1 also links the phosphorylation machinery to the ubiquitin-proteasome pathway to ensure prompt and efficient proteolysis of β-catenin in response to external signals. SCF(FWD1) may be critical for tumor development and suppression through regulation of β-catenin protein stability.

元の言語英語
ページ(範囲)2401-2410
ページ数10
ジャーナルEMBO Journal
18
発行部数9
DOI
出版物ステータス出版済み - 5 4 1999

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F-Box Proteins
Proteolysis
Catenins
Ubiquitin
Ubiquitination
Phosphorylation
SKP Cullin F-Box Protein Ligases
Glycogen Synthase Kinase 3
Adenomatous Polyposis Coli Protein
Tumor Suppressor Proteins
Mutation
Adenomatous Polyposis Coli
Deregulation
Protein Stability
Cell adhesion
Proteasome Endopeptidase Complex
Cadherins
Ligases
Cell Adhesion

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

これを引用

Kitagawa, M., Hatakeyama, S., Shirane, M., Matsumoto, M., Ishida, N., Hattori, K., ... Nakayama, K. (1999). An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of β-catenin. EMBO Journal, 18(9), 2401-2410. https://doi.org/10.1093/emboj/18.9.2401

An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of β-catenin. / Kitagawa, Masatoshi; Hatakeyama, Shigetsugu; Shirane, Michiko; Matsumoto, Masaki; Ishida, Noriko; Hattori, Kimihiko; Nakamichi, Ikuo; Kikuchi, Akira; Nakayama, Kei Ichi; Nakayama, Keiko.

:: EMBO Journal, 巻 18, 番号 9, 04.05.1999, p. 2401-2410.

研究成果: ジャーナルへの寄稿記事

Kitagawa, M, Hatakeyama, S, Shirane, M, Matsumoto, M, Ishida, N, Hattori, K, Nakamichi, I, Kikuchi, A, Nakayama, KI & Nakayama, K 1999, 'An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of β-catenin', EMBO Journal, 巻. 18, 番号 9, pp. 2401-2410. https://doi.org/10.1093/emboj/18.9.2401
Kitagawa M, Hatakeyama S, Shirane M, Matsumoto M, Ishida N, Hattori K その他. An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of β-catenin. EMBO Journal. 1999 5 4;18(9):2401-2410. https://doi.org/10.1093/emboj/18.9.2401
Kitagawa, Masatoshi ; Hatakeyama, Shigetsugu ; Shirane, Michiko ; Matsumoto, Masaki ; Ishida, Noriko ; Hattori, Kimihiko ; Nakamichi, Ikuo ; Kikuchi, Akira ; Nakayama, Kei Ichi ; Nakayama, Keiko. / An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of β-catenin. :: EMBO Journal. 1999 ; 巻 18, 番号 9. pp. 2401-2410.
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abstract = "β-catenin plays an essential role in the Wingless/Wnt signaling cascade and is a component of the cadherin cell adhesion complex. Deregulation of β-catenin accumulation as a result of mutations in adenomatous polyposis coli (APC) tumor suppressor protein is believed to initiate colorectal neoplasia. β-catenin levels are regulated by the ubiquitin-dependent proteolysis system and β-catenin ubiquitination is preceded by phosphorylation of its N-terminal region by the glycogen synthase kinase-3β (GSK-3β)/Axin kinase complex. Here we show that FWD1 (the mouse homologue of Slimb/βTrCP), an F-box/WD40-repeat protein, specifically formed a multi-molecular complex with β-catenin, Axin, GSK-3β and APC. Mutations at the signal-induced phosphorylation site of β-catenin inhibited its association with FWD1, FWD1 facilitated ubiquitination and promoted degradation of β-catenin, resulting in reduced cytoplasmic β-catenin levels. In contrast, a dominant-negative mutant form of FWD1 inhibited the ubiquitination process and stabilized β-catenin. These results suggest that the Skp1/Cullin/F-box protein FWD1 (SCF(FWD1))-ubiquitin ligase complex is involved in β-catenin ubiquitination and that FWD1 serves as an intracellular receptor for phosphorylated β-catenin. FWD1 also links the phosphorylation machinery to the ubiquitin-proteasome pathway to ensure prompt and efficient proteolysis of β-catenin in response to external signals. SCF(FWD1) may be critical for tumor development and suppression through regulation of β-catenin protein stability.",
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