An immune-responsive Serpin regulates the melanization cascade in Drosophila

Ennio De Gregorio, Sung Jun Han, Won Jae Lee, Min Ji Baek, Tsukasa Osaki, Shun Ichiro Kawabata, Bok Leul Lee, Sadaaki Iwanaga, Bruno Lemaitre, Paul T. Brey

研究成果: ジャーナルへの寄稿記事

243 引用 (Scopus)

抄録

In arthropods, the melanization reaction is associated with multiple host defense mechanism leading to the sequestration and killing of invading microorganisms. Arthropod melanization is controlled by a cascade of serine proteases that ultimately activates the enzyme prophenoloxidase (PPO), which, in turn, catalyzes the synthesis of melanin. Here we report the biochemical and genetic characterization of a Drosophila serine protease inhibitor protein, Serpin-27A, which regulates the melanization cascade through the specific inhibition of the terminal protease prophenoloxidase-activating enzyme. Our data demonstrate that Serpin-27A is required to restrict the phenoloxidase activity to the site of injury or infection, preventing the insect from excessive melanization.

元の言語英語
ページ(範囲)581-592
ページ数12
ジャーナルDevelopmental Cell
3
発行部数4
DOI
出版物ステータス出版済み - 10 1 2002

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Serpins
Arthropods
Drosophila
Serine Proteinase Inhibitors
Monophenol Monooxygenase
Melanins
Serine Proteases
Microorganisms
Insects
Molecular Biology
Peptide Hydrolases
Wounds and Injuries
Enzymes
Infection
Proteins
pro-phenoloxidase
prephenoloxidase-activating enzyme

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

これを引用

De Gregorio, E., Han, S. J., Lee, W. J., Baek, M. J., Osaki, T., Kawabata, S. I., ... Brey, P. T. (2002). An immune-responsive Serpin regulates the melanization cascade in Drosophila. Developmental Cell, 3(4), 581-592. https://doi.org/10.1016/S1534-5807(02)00267-8

An immune-responsive Serpin regulates the melanization cascade in Drosophila. / De Gregorio, Ennio; Han, Sung Jun; Lee, Won Jae; Baek, Min Ji; Osaki, Tsukasa; Kawabata, Shun Ichiro; Lee, Bok Leul; Iwanaga, Sadaaki; Lemaitre, Bruno; Brey, Paul T.

:: Developmental Cell, 巻 3, 番号 4, 01.10.2002, p. 581-592.

研究成果: ジャーナルへの寄稿記事

De Gregorio, E, Han, SJ, Lee, WJ, Baek, MJ, Osaki, T, Kawabata, SI, Lee, BL, Iwanaga, S, Lemaitre, B & Brey, PT 2002, 'An immune-responsive Serpin regulates the melanization cascade in Drosophila', Developmental Cell, 巻. 3, 番号 4, pp. 581-592. https://doi.org/10.1016/S1534-5807(02)00267-8
De Gregorio, Ennio ; Han, Sung Jun ; Lee, Won Jae ; Baek, Min Ji ; Osaki, Tsukasa ; Kawabata, Shun Ichiro ; Lee, Bok Leul ; Iwanaga, Sadaaki ; Lemaitre, Bruno ; Brey, Paul T. / An immune-responsive Serpin regulates the melanization cascade in Drosophila. :: Developmental Cell. 2002 ; 巻 3, 番号 4. pp. 581-592.
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AU - Lee, Won Jae

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AU - Osaki, Tsukasa

AU - Kawabata, Shun Ichiro

AU - Lee, Bok Leul

AU - Iwanaga, Sadaaki

AU - Lemaitre, Bruno

AU - Brey, Paul T.

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