抄録
In arthropods, the melanization reaction is associated with multiple host defense mechanism leading to the sequestration and killing of invading microorganisms. Arthropod melanization is controlled by a cascade of serine proteases that ultimately activates the enzyme prophenoloxidase (PPO), which, in turn, catalyzes the synthesis of melanin. Here we report the biochemical and genetic characterization of a Drosophila serine protease inhibitor protein, Serpin-27A, which regulates the melanization cascade through the specific inhibition of the terminal protease prophenoloxidase-activating enzyme. Our data demonstrate that Serpin-27A is required to restrict the phenoloxidase activity to the site of injury or infection, preventing the insect from excessive melanization.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 581-592 |
| ページ数 | 12 |
| ジャーナル | Developmental Cell |
| 巻 | 3 |
| 発行部数 | 4 |
| DOI | |
| 出版物ステータス | 出版済み - 10 1 2002 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Developmental Biology
- Cell Biology
これを引用
An immune-responsive Serpin regulates the melanization cascade in Drosophila. / De Gregorio, Ennio; Han, Sung Jun; Lee, Won Jae; Baek, Min Ji; Osaki, Tsukasa; Kawabata, Shun Ichiro; Lee, Bok Leul; Iwanaga, Sadaaki; Lemaitre, Bruno; Brey, Paul T.
：: Developmental Cell, 巻 3, 番号 4, 01.10.2002, p. 581-592.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - An immune-responsive Serpin regulates the melanization cascade in Drosophila
AU - De Gregorio, Ennio
AU - Han, Sung Jun
AU - Lee, Won Jae
AU - Baek, Min Ji
AU - Osaki, Tsukasa
AU - Kawabata, Shun Ichiro
AU - Lee, Bok Leul
AU - Iwanaga, Sadaaki
AU - Lemaitre, Bruno
AU - Brey, Paul T.
PY - 2002/10/1
Y1 - 2002/10/1
N2 - In arthropods, the melanization reaction is associated with multiple host defense mechanism leading to the sequestration and killing of invading microorganisms. Arthropod melanization is controlled by a cascade of serine proteases that ultimately activates the enzyme prophenoloxidase (PPO), which, in turn, catalyzes the synthesis of melanin. Here we report the biochemical and genetic characterization of a Drosophila serine protease inhibitor protein, Serpin-27A, which regulates the melanization cascade through the specific inhibition of the terminal protease prophenoloxidase-activating enzyme. Our data demonstrate that Serpin-27A is required to restrict the phenoloxidase activity to the site of injury or infection, preventing the insect from excessive melanization.
AB - In arthropods, the melanization reaction is associated with multiple host defense mechanism leading to the sequestration and killing of invading microorganisms. Arthropod melanization is controlled by a cascade of serine proteases that ultimately activates the enzyme prophenoloxidase (PPO), which, in turn, catalyzes the synthesis of melanin. Here we report the biochemical and genetic characterization of a Drosophila serine protease inhibitor protein, Serpin-27A, which regulates the melanization cascade through the specific inhibition of the terminal protease prophenoloxidase-activating enzyme. Our data demonstrate that Serpin-27A is required to restrict the phenoloxidase activity to the site of injury or infection, preventing the insect from excessive melanization.
UR - http://www.scopus.com/inward/record.url?scp=18644376155&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18644376155&partnerID=8YFLogxK
U2 - 10.1016/S1534-5807(02)00267-8
DO - 10.1016/S1534-5807(02)00267-8
M3 - Article
C2 - 12408809
AN - SCOPUS:18644376155
VL - 3
SP - 581
EP - 592
JO - Developmental Cell
JF - Developmental Cell
SN - 1534-5807
IS - 4
ER -