An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease

Sayuri Hara-Kuge, Tomonobu Nishihara, Tomoki Matsuda, Tomohiro Kitazono, Takayuki Teramoto, Takeharu Nagai, Takeshi Ishihara

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Sensory processing is regulated by the coordinated excitation and inhibition of neurons in neuronal circuits. The analysis of neuronal activities has greatly benefited from the recent development of genetically encoded Ca2+ indicators (GECIs). These molecules change their fluorescence intensities or colours in response to changing levels of Ca2+ and can, therefore, be used to sensitively monitor intracellular Ca2+ concentration, which enables the detection of neuronal excitation, including action potentials. These GECIs were developed to monitor increases in Ca2+ concentration; therefore, neuronal inhibition cannot be sensitively detected by these GECIs. To overcome this difficulty, we hypothesised that an inverse-type of GECI, whose fluorescence intensity increases as Ca2+ levels decrease, could sensitively monitor reducing intracellular Ca2+ concentrations. We, therefore, developed a Ca2+ indicator named inverse-pericam 2.0 (IP2.0) whose fluorescent intensity decreases 25-fold upon Ca2+ binding in vitro. Using IP2.0, we successfully detected putative neuronal inhibition by monitoring the decrease in intracellular Ca2+ concentration in AWCON and ASEL neurons in Caenorhabditis elegans. Therefore, IP2.0 is a useful tool for studying neuronal inhibition and for the detailed analysis of neuronal activities in vivo.

元の言語英語
記事番号e0194707
ジャーナルPloS one
13
発行部数4
DOI
出版物ステータス出版済み - 4 1 2018

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Caenorhabditis elegans
Neurons
Fluorescence
calcium
Action Potentials
Color
Molecules
Networks (circuits)
Monitoring
Processing
monitoring
neurons
fluorescence
action potentials
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease. / Hara-Kuge, Sayuri; Nishihara, Tomonobu; Matsuda, Tomoki; Kitazono, Tomohiro; Teramoto, Takayuki; Nagai, Takeharu; Ishihara, Takeshi.

:: PloS one, 巻 13, 番号 4, e0194707, 01.04.2018.

研究成果: ジャーナルへの寄稿記事

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AU - Matsuda, Tomoki

AU - Kitazono, Tomohiro

AU - Teramoto, Takayuki

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