An improved method for assay of cholesterol 7α-hydroxylase activity

Tadashi Ogishima, Kyuichiro Okuda

研究成果: ジャーナルへの寄稿記事

89 引用 (Scopus)

抄録

An improved assay method for cholesterol 7α-hydroxylase which is accurate, sensitive and yet still simple is described. The method consists of two parts: the first part is cholesterol 7α-hydroxylation in liver microsomes utilizing cholesterol in situ as the substrate, and the second part is conversion of the product, 7α-hydroxycholesterol, into 7α-hydroxy-4-cholesten-3-one having an intense absorption at 240 nm by the action of cholesterol oxidase. The converted sterol is then analyzed by high-performance liquid chromatography. During the second enzyme reaction, the first enzyme reaction is halted and further metabolism of the product is prevented. In consequence, the method had more than 10-fold increase in the sensitivity than the previous one.

元の言語英語
ページ(範囲)228-232
ページ数5
ジャーナルAnalytical Biochemistry
158
発行部数1
DOI
出版物ステータス出版済み - 10 1986

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Cholesterol 7-alpha-Hydroxylase
Assays
Cholesterol Oxidase
Cholesterol
Hydroxycholesterols
Hydroxylation
High performance liquid chromatography
Sterols
Enzymes
Metabolism
Liver
Liver Microsomes
Substrates
High Pressure Liquid Chromatography
7 alpha-hydroxy-4-cholesten-3-one

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

An improved method for assay of cholesterol 7α-hydroxylase activity. / Ogishima, Tadashi; Okuda, Kyuichiro.

:: Analytical Biochemistry, 巻 158, 番号 1, 10.1986, p. 228-232.

研究成果: ジャーナルへの寄稿記事

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