An improved method for isolation of epithelial and stromal cells from the human endometrium

Ayako Masuda, Noriko Katoh, Kazuhiko Nakabayashi, Kiyoko Kato, Kenzo Sonoda, Mari Kitade, Satoru Takeda, Kenichiro Hata, Junko Tomikawa

研究成果: Contribution to journalArticle査読

17 被引用数 (Scopus)

抄録

We aimed to improve the efficiency of isolating endometrial epithelial and stromal cells (EMECs and EMSCs) from the human endometrium. We revealed by immunohistochemical staining that the large tissue fragments remaining after collagenase treatment, which are usually discarded after the first filtration in the conventional protocol, consisted of glandular epithelial and stromal cells. Therefore, we established protease treatment and cell suspension conditions to dissociate single cells from the tissue fragments and isolated epithelial (EPCAM-positive) and stromal (CD13-positive) cells by fluorescenceactivated cell sorting. Four independent experiments showed that, on average, 1.2 × 106 of EMECs and 2.8 × 106 EMSCs were isolated from one hysterectomy specimen. We confirmed that the isolated cells presented transcriptomic features highly similar to those of epithelial and stromal cells obtained by the conventional method. Our improved protocol facilitates future studies to better understand the molecular mechanisms underlying the dynamic changes of the endometrium during the menstrual cycle.

本文言語英語
ページ(範囲)213-218
ページ数6
ジャーナルJournal of Reproduction and Development
62
2
DOI
出版ステータス出版済み - 4 22 2016
外部発表はい

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

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