TY - JOUR
T1 - An improved mounting method for observation of thick specimens using confocal microscopy
AU - Kagayama, M.
AU - Sasano, Y.
AU - Hirata, M.
AU - Mizoguchi, I.
AU - Takahashi, I.
N1 - Funding Information:
We thank Mr. M. Eguchi and Mr. Y. Mikami for their technical assistance. This study was supported in part by grants-in-aid (05557077, 0757282, and 07557283) from the Ministry of Science, Education and Culture of Japan.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1996/9
Y1 - 1996/9
N2 - When applying aqueous media to mount thick specimens, air bubbles occur frequently between coverslips and slide glasses. This results in an increase of background signal by confocal microscopy. Using Spurr's resin as a mounting medium, we could observe thick specimens with oil immersion objective lens without the use of coverslips, then avoid air bubbles near the specimen. Fading of the fluorescence appeared to be less in specimens mounted by the resin than in specimens mounted by aqueous media. Further, the new method increased depth of field so that more planes of specimens could be analyzed.
AB - When applying aqueous media to mount thick specimens, air bubbles occur frequently between coverslips and slide glasses. This results in an increase of background signal by confocal microscopy. Using Spurr's resin as a mounting medium, we could observe thick specimens with oil immersion objective lens without the use of coverslips, then avoid air bubbles near the specimen. Fading of the fluorescence appeared to be less in specimens mounted by the resin than in specimens mounted by aqueous media. Further, the new method increased depth of field so that more planes of specimens could be analyzed.
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U2 - 10.3109/10520299609117165
DO - 10.3109/10520299609117165
M3 - Article
C2 - 8896795
AN - SCOPUS:0029903779
SN - 1052-0295
VL - 71
SP - 231
EP - 233
JO - Biotechnic and Histochemistry
JF - Biotechnic and Histochemistry
IS - 5
ER -