An in vitro system for infection with hepatitis B virus that uses primary human fetal hepatocytes

T. Ochiya, T. Tsurimoto, K. Ueda, K. Okubo, M. Shiozawa, K. Matsubara

研究成果: Contribution to journalArticle査読

75 被引用数 (Scopus)

抄録

An in vitro culture of human fetal hepatocytes has been employed for infection by hepatitis B virus (HBV) virions that are produced by an established human hepatoma cell line, HB 611. HBV surface antigen and e antigen were released into the medium 3-4 days after infection, and production continued thereafter. RNA synthesis with similar kinetics was observed. Viral DNA replication started 2 days after infection, and replicative HBV DNA that included relaxed circles, single-stranded minus strands, and closed circles accumulated during 16 days of incubation. Immunofluorescent study using fluorescein isothiocyanate-labeled rabbit antisera directed against HBV core antigen revealed that this antigen is present in the nuclei in 12% of the infected cells. Particles containing HBV DNA were detected in the culture medium and were infectious. Thus, this in vitro infection system closely mimics infection in vivo and it allows detailed studies on early events associated with human HBV entry into cells and subsequent replication and integration.

本文言語英語
ページ(範囲)1875-1879
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
86
6
DOI
出版ステータス出版済み - 1989

All Science Journal Classification (ASJC) codes

  • 一般

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