An in vitro three-dimensional organotypic model to analyze peripancreatic fat invasion in pancreatic cancer: A culture system based on collagen gel embedding

研究成果: 著書/レポートタイプへの貢献

抄録

Three-dimensional culture systems reflect biological environments better than conventional two-dimensional culture. Additionally, three-dimensional culture is a strong experimental tool to analyze direct interactions between cancer cells and stromal cells in vitro. Herein, we describe protocols for an organotypic fat invasion model that is a novel culturing system mimicking the extrapancreatic invasion of pancreatic adenocarcinoma (PDAC). This novel model is based on the collagen I gel embedding method and enables us to analyze the functional and histological interactions between cancer cells and adipose tissue.

元の言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版者Humana Press Inc.
ページ135-141
ページ数7
DOI
出版物ステータス出版済み - 1 1 2019

出版物シリーズ

名前Methods in Molecular Biology
1882
ISSN(印刷物)1064-3745

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Pancreatic Neoplasms
Collagen
Gels
Fats
Stromal Cells
Adipose Tissue
Neoplasms
Adenocarcinoma
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

An in vitro three-dimensional organotypic model to analyze peripancreatic fat invasion in pancreatic cancer : A culture system based on collagen gel embedding. / Okumura, Takashi; Ouchida, Kenoki; Nakamura, Masafumi.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 135-141 (Methods in Molecular Biology; 巻 1882).

研究成果: 著書/レポートタイプへの貢献

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