An inhibitory effect of actin on casein kinase II activity in vitro

Atsushi Karino, Shin Tanoue, Motoko Fukuda, Takeshi Nakamura, Kenzo Ohtsuki

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)


The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of α-monomeric CK-II (CK-IIα) as well as oligomeric CK-II (α2β2) in a dose-dependent manner, but has no effect on the activities of the two other kinases; and (ii) actin-induced inhibition of CK-II activity is due to the binding of actin to the α-subunit of CK-II and is non-competitive with its phosphate acceptors. In addition, it is demonstrated that actin binds directly to CK-II: both actin and CK-II are coprecipitated by anti-serum against Drosophila CK-IIβ or by specific IgG against Ascaris suum muscle actin. The results presented here suggest that actin can suppress CK-II-mediated signal transduction.

ジャーナルFEBS Letters
出版ステータス出版済み - 12 2 1996

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学


「An inhibitory effect of actin on casein kinase II activity in vitro」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。