TY - JOUR
T1 - An integrated enzyme-linked immunosorbent assay system with an organic light-emitting diode and a charge-coupled device for fluorescence detection
AU - Nakajima, Hizuru
AU - Okuma, Yukiko
AU - Morioka, Kazuhiro
AU - Miyake, Mayo
AU - Hemmi, Akihide
AU - Tobita, Tatsuya
AU - Yahiro, Masayuki
AU - Yokoyama, Daisuke
AU - Adachi, Chihaya
AU - Soh, Nobuaki
AU - Nakano, Koji
AU - Xue, Shuhua
AU - Zeng, Hulie
AU - Uchiyama, Katsumi
AU - Imato, Toshihiko
PY - 2011/10
Y1 - 2011/10
N2 - A fluorescence detection system for a microfluidic device using an organic light-emitting diode (OLED) as the excitation light source and a charge-coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique. The OLED produced a green luminescence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm 2 was 13 μW (1.7 mW/cm 2). The fluorescence detection system consisted of the OLED device, two band-pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow-based enzyme-linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit (S/N=3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96-well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced.
AB - A fluorescence detection system for a microfluidic device using an organic light-emitting diode (OLED) as the excitation light source and a charge-coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique. The OLED produced a green luminescence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm 2 was 13 μW (1.7 mW/cm 2). The fluorescence detection system consisted of the OLED device, two band-pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow-based enzyme-linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit (S/N=3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96-well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced.
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U2 - 10.1002/jssc.201100429
DO - 10.1002/jssc.201100429
M3 - Article
C2 - 21898810
AN - SCOPUS:80054982463
VL - 34
SP - 2906
EP - 2912
JO - Journal of Separation Science
JF - Journal of Separation Science
SN - 1615-9306
IS - 20
ER -