An intermediate of ubiquinone biosynthesis exists in the microsomal fraction of hepG2 cells

Dongchon Rang, Koichiro Takeshige, Shigeki Minakami

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

We analyzed lipids extracted from human hepatoma HepG2 cells using a high performance liquid chromatograph equipped with a reversed phase column and found a compound with a mass spectrum showing certain diagnostic ion framgents of 1-methoxy-5-polyprenyl-phenol, a known intermediate of ubiquinone biosynthesis. Universally radiolabeled [14C] -p-hydroxybenzoate, a precursor of ubiquinone, was incorporated into the compound on incubation with the cells, suggesting that the compound is a precursor of ubiquinone. The presence of the compound in the microsomal fraction of HepG2 cells was not due to contamination by the mitochondrial fraction because the activity of succinate-cytochrome c reductase in the microsomal fraction was below 1% of that in the mitochondrial fraction, whereas the contents of ubiquinone and the compound in the former were 4.6 and 7.8% of those in the latter, respectively. These results support the hypothesis that ubiquinone biosynthesis might occur in microsomes as well as mitochondria.

本文言語英語
ページ(範囲)893-895
ページ数3
ジャーナルJournal of biochemistry
108
6
DOI
出版ステータス出版済み - 12 1990

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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