An intracellular kinase signal-responsive gene carrier for disordered cell-specific gene therapy

Jun Oishi, Kenji Kawamura, Jeong Hun Kang, Kota Kodama, Tatsuhiko Sonoda, Masaharu Murata, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

45 引用 (Scopus)

抄録

We have previously reported artificial gene-regulation systems responding to cyclic AMP-dependent protein kinase (PKA) using cationic polymer. This cationic polymer (PAK) was a graft-type polymer with an oligopeptide that is a substrate for PKA and could regulate gene-expression in a cell-free system. In the present study, we carried out a detailed characterization of the PAK-DNA complex (AFM observation and DLS measurement) and tried to apply this polymer to living cells. In the unstimulated NIH 3T3 cells, transfection of the PAK-DNA complex showed no expression of the delivered gene. This means that PAK formed a stable complex with DNA in the normal cells to totally suppress gene expression. In contrast, significant expression was seen when the PAK-DNA complex was delivered to forskolin-treated cells. Thus, activated PKA disintegrates the complexes even in living cells, resulting in gene expression. Our results indicate that this type of intracellular signal-responsive polymer will be useful for the cell-specific release of genes.

元の言語英語
ページ(範囲)431-436
ページ数6
ジャーナルJournal of Controlled Release
110
発行部数2
DOI
出版物ステータス出版済み - 1 10 2006

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Genetic Therapy
Polymers
Phosphotransferases
Gene Expression
Genes
DNA
Protein Kinases
Synthetic Genes
NIH 3T3 Cells
Oligopeptides
Cell-Free System
Colforsin
Cyclic AMP-Dependent Protein Kinases
Transfection
Observation
Transplants

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

これを引用

An intracellular kinase signal-responsive gene carrier for disordered cell-specific gene therapy. / Oishi, Jun; Kawamura, Kenji; Kang, Jeong Hun; Kodama, Kota; Sonoda, Tatsuhiko; Murata, Masaharu; Niidome, Takuro; Katayama, Yoshiki.

:: Journal of Controlled Release, 巻 110, 番号 2, 10.01.2006, p. 431-436.

研究成果: ジャーナルへの寄稿記事

Oishi, Jun ; Kawamura, Kenji ; Kang, Jeong Hun ; Kodama, Kota ; Sonoda, Tatsuhiko ; Murata, Masaharu ; Niidome, Takuro ; Katayama, Yoshiki. / An intracellular kinase signal-responsive gene carrier for disordered cell-specific gene therapy. :: Journal of Controlled Release. 2006 ; 巻 110, 番号 2. pp. 431-436.
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AU - Murata, Masaharu

AU - Niidome, Takuro

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