An on-membrane quantitative analysis system for glycyrrhizin in licorice roots and traditional Chinese medicines

Osamu Morinaga, Ayako Fujino, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

An on-membrane quantitative analysis system has been developed for determining glycyrrhizin (GC) in licorice roots and traditional Chinese medicines. A GC standard and the extracts of licorice roots and traditional Chinese medicines were applied to a polyethersulfone (PES) membrane and were developed by acetonitrile/water/formic acid (45:55:2, by volume), then treated with a NaIO4 solution followed by bovine serum albumin (BSA), resulting in a GC-BSA conjugate on a PES membrane. Anti-GC monoclonal antibody was bound and then a second antibody labeled with peroxidase directed against the first antibody. Finally a substrate reacted with the enzyme and gave staining. The stained membrane was scanned and coloring spots were analyzed quantitatively using graphic analysis by NIH Image software, indicating at least 0.5 μg of GC was clearly detectable. GC can be analyzed quantitatively between 1.0 and 8.0 μg.

元の言語英語
ページ(範囲)668-672
ページ数5
ジャーナルAnalytical and Bioanalytical Chemistry
383
発行部数4
DOI
出版物ステータス出版済み - 10 1 2005

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Glycyrrhizic Acid
Glycyrrhiza
Chinese Traditional Medicine
Medicine
Membranes
Chemical analysis
formic acid
Bovine Serum Albumin
Antibodies
Coloring
Peroxidase
Software
Monoclonal Antibodies
Staining and Labeling
Water
Substrates
Enzymes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

これを引用

An on-membrane quantitative analysis system for glycyrrhizin in licorice roots and traditional Chinese medicines. / Morinaga, Osamu; Fujino, Ayako; Tanaka, Hiroyuki; Shoyama, Yukihiro.

:: Analytical and Bioanalytical Chemistry, 巻 383, 番号 4, 01.10.2005, p. 668-672.

研究成果: ジャーナルへの寄稿記事

Morinaga, Osamu ; Fujino, Ayako ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / An on-membrane quantitative analysis system for glycyrrhizin in licorice roots and traditional Chinese medicines. :: Analytical and Bioanalytical Chemistry. 2005 ; 巻 383, 番号 4. pp. 668-672.
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