An unique CD4+CD8+ intestinal intraepithelial lymphocyte specific for DnaK (Escherichia coli HSP70) may be selected by intestinal microflora of rats

Yuki Kimura, Tetsu Sakai, Makoto Takeuchi, Yoshihiro Matsumoto, Kouichi Watanabe, Masakatsu Yuuki, Toshihiko Takada, Yasunobu Yoshikai

    研究成果: Contribution to journalArticle査読

    7 被引用数 (Scopus)

    抄録

    We have previously shown an age-associated increase in unique CD4+CD8+ intestinal intraepithelial lymphocytes (i-IEL) in rats. To elucidate the potential causes of the increase in CD4+CD8+ i-IEL with age, we analyzed the specificity of the CD4+CD8+ i-IEL and influence of intestinal microflora on the increase in this subset in aged rats. The purified CD4+CD8+ i-IEL proliferated in response to DnaK [Escherichia coli (E. coli) HSP70] in the presence of mitomycin-c (MMC)-treated syngeneic spleen cells. The proportion of CD4+CD8+ T cells in whole i-IEL were significantly increased in aged rats fed commercial (CL-2) diet but not in those fed home-made (purified) diet under conventional condition. No CD4+CD8+ i-IEL were detected in aged rats under germfree condition, irrespective of diet feeding. A larger number of Enterobacteriaceae, especially E. coli, were detected in the intestinal contents and feces from aged rats with CD4+CD8+ i-IEL compared with those from aged rats fed without CD4+CD8+ i-IEL. The unique CD4+CD8+ i-IEL population specific for E. coli HSP may be associated with long term exposure to intestinal E. coli in aged rats.

    本文言語英語
    ページ(範囲)550-566
    ページ数17
    ジャーナルImmunobiology
    196
    5
    出版ステータス出版済み - 3 1 1997

    All Science Journal Classification (ASJC) codes

    • 免疫アレルギー学
    • 免疫学
    • 血液学

    フィンガープリント

    「An unique CD4<sup>+</sup>CD8<sup>+</sup> intestinal intraepithelial lymphocyte specific for DnaK (Escherichia coli HSP70) may be selected by intestinal microflora of rats」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル