Analysis of cell viability and differential activity of mouse hepatocytes under 3D and 2D culture in agarose gel

Hirohiko Ise, Seiji Takashima, Masato Nagaoka, Anwarul Ferdous, Toshihiro Akaike

研究成果: Contribution to journalArticle査読

16 被引用数 (Scopus)

抄録

Three-dimensional (3D) and two-dimensional (2D) cultures of hepatocytes in various concentrations (0.3-0.7%) of agarose gel revealed that the hepatocytes under 3D cultures in 0.3% agarose gel possess long-term (> 3 weeks) viability, significant self-assembly to form tissue like aggregates, low lactate dehydrogenase release and high albumin synthesis. These were in contrast to 2D culture of hepatocytes. Our results suggest that the 3D culture of hepatocytes in agarose gel favors aggregate formation of functionally active cells and would be useful for liver transplantation as well as to analyze hepatocytes biology.

本文言語英語
ページ(範囲)209-213
ページ数5
ジャーナルBiotechnology letters
21
3
DOI
出版ステータス出版済み - 1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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