TY - CHAP
T1 - Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis
AU - Takeshita, Nobuo
AU - Takano-Yamamoto, Teruko
N1 - Funding Information:
We would like to gratefully thank Dr. Wei Jiang for her help to perform this study. We would also like to thank the Biomedical Research Unit of Tohoku University Hospital for technical equipment support. This study was supported by a Grant-in-Aid for Scientific Research (18K09828 and 21K10156 to NT and 15H05048 to TT-Y) from the Japan Society for the Promotion of Science.
Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Chemotaxis is a directed migration of cells in response to a gradient of extracellular molecules called chemoattractants. Development, growth, remodeling, and fracture healing of bones are advanced through intramembranous osteogenesis. Chemotaxis of preosteoblasts toward future bone formation sites observed in the early stage of intramembranous osteogenesis is a critical cellular process for normal bone formation. However, molecular biological mechanisms of the chemotaxis of preosteoblasts are not fully understood. We have recently clarified, for the first time, the critical role of the cellular communication network factor 2 (CCN2)/connective tissue growth factor (CTGF)-integrin α5-Ras axis for chemotaxis of preosteoblasts during new bone formation through intramembranous osteogenesis. In this chapter, we describe in detail the procedures of the in vivo and in vitro assays to investigate the chemotactic property of CCN2/CTGF and its underlying molecular biological mechanisms during intramembranous osteogenesis.
AB - Chemotaxis is a directed migration of cells in response to a gradient of extracellular molecules called chemoattractants. Development, growth, remodeling, and fracture healing of bones are advanced through intramembranous osteogenesis. Chemotaxis of preosteoblasts toward future bone formation sites observed in the early stage of intramembranous osteogenesis is a critical cellular process for normal bone formation. However, molecular biological mechanisms of the chemotaxis of preosteoblasts are not fully understood. We have recently clarified, for the first time, the critical role of the cellular communication network factor 2 (CCN2)/connective tissue growth factor (CTGF)-integrin α5-Ras axis for chemotaxis of preosteoblasts during new bone formation through intramembranous osteogenesis. In this chapter, we describe in detail the procedures of the in vivo and in vitro assays to investigate the chemotactic property of CCN2/CTGF and its underlying molecular biological mechanisms during intramembranous osteogenesis.
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U2 - 10.1007/978-1-0716-2744-0_16
DO - 10.1007/978-1-0716-2744-0_16
M3 - Chapter
C2 - 36370354
AN - SCOPUS:85141668124
T3 - Methods in Molecular Biology
SP - 237
EP - 253
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -