抄録
Endometrial gene expression is primarily regulated by the ovarian steroids and pregnancy recognition factors. This study was aimed to characterize differential expression genes (DEGs) in bovine endometrium together with the analysis of their promoter region. Bovine uteri at follicular stage (FS), luteal stage (LS), and implantation stage (IS) at Day 18 of pregnancy were collected. Total RNA extracted and prepared cDNA were then subjected to high-throughput sequencing. For promoter analysis, 1 kb upstream promoter region of each DEG was analyzed. The numbers of highly expressed DEGs were 496 and 597 at FS and LS, respectively. When compared the gene expression of IS with LS, 383 and 346 DEGs showed higher and lower expression at IS, respectively. It was also observed that 20–30 transcription factors (TFs) were included in each DEGs. In addition, promoter analyses estimated 150–160 TFs for each stage. DLX4 and interferon regulatory factor 4 (IRF4) at FS, and IRF5, IRF9, STAT1, and STAT2 at IS were in common to DEGs and estimated TFs, respectively. This study highlighted potential molecular mechanisms controlling endometrial function during estrus cycle and IS, which will further guide to better understand the endometrial functions in future studies.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 1609-1621 |
| ページ数 | 13 |
| ジャーナル | Animal Science Journal |
| 巻 | 89 |
| 発行部数 | 11 |
| DOI | |
| 出版物ステータス | 出版済み - 11 1 2018 |
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All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences(all)
これを引用
Analysis of differentially expressed genes and the promoters in bovine endometrium throughout estrus cycle and early pregnancy. / Musavi, Sayed A.A.; Yamashita, Seiya; Fujihara, Taisuke; Masaka, Hironori; Islam, Md Rashedul; Kim, Sangwan; Gotoh, Takafumi; Kawahara, Manabu; Tashiro, Kosuke; Yamauchi, Nobuhiko.
：: Animal Science Journal, 巻 89, 番号 11, 01.11.2018, p. 1609-1621.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Analysis of differentially expressed genes and the promoters in bovine endometrium throughout estrus cycle and early pregnancy
AU - Musavi, Sayed A.A.
AU - Yamashita, Seiya
AU - Fujihara, Taisuke
AU - Masaka, Hironori
AU - Islam, Md Rashedul
AU - Kim, Sangwan
AU - Gotoh, Takafumi
AU - Kawahara, Manabu
AU - Tashiro, Kosuke
AU - Yamauchi, Nobuhiko
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Endometrial gene expression is primarily regulated by the ovarian steroids and pregnancy recognition factors. This study was aimed to characterize differential expression genes (DEGs) in bovine endometrium together with the analysis of their promoter region. Bovine uteri at follicular stage (FS), luteal stage (LS), and implantation stage (IS) at Day 18 of pregnancy were collected. Total RNA extracted and prepared cDNA were then subjected to high-throughput sequencing. For promoter analysis, 1 kb upstream promoter region of each DEG was analyzed. The numbers of highly expressed DEGs were 496 and 597 at FS and LS, respectively. When compared the gene expression of IS with LS, 383 and 346 DEGs showed higher and lower expression at IS, respectively. It was also observed that 20–30 transcription factors (TFs) were included in each DEGs. In addition, promoter analyses estimated 150–160 TFs for each stage. DLX4 and interferon regulatory factor 4 (IRF4) at FS, and IRF5, IRF9, STAT1, and STAT2 at IS were in common to DEGs and estimated TFs, respectively. This study highlighted potential molecular mechanisms controlling endometrial function during estrus cycle and IS, which will further guide to better understand the endometrial functions in future studies.
AB - Endometrial gene expression is primarily regulated by the ovarian steroids and pregnancy recognition factors. This study was aimed to characterize differential expression genes (DEGs) in bovine endometrium together with the analysis of their promoter region. Bovine uteri at follicular stage (FS), luteal stage (LS), and implantation stage (IS) at Day 18 of pregnancy were collected. Total RNA extracted and prepared cDNA were then subjected to high-throughput sequencing. For promoter analysis, 1 kb upstream promoter region of each DEG was analyzed. The numbers of highly expressed DEGs were 496 and 597 at FS and LS, respectively. When compared the gene expression of IS with LS, 383 and 346 DEGs showed higher and lower expression at IS, respectively. It was also observed that 20–30 transcription factors (TFs) were included in each DEGs. In addition, promoter analyses estimated 150–160 TFs for each stage. DLX4 and interferon regulatory factor 4 (IRF4) at FS, and IRF5, IRF9, STAT1, and STAT2 at IS were in common to DEGs and estimated TFs, respectively. This study highlighted potential molecular mechanisms controlling endometrial function during estrus cycle and IS, which will further guide to better understand the endometrial functions in future studies.
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U2 - 10.1111/asj.13091
DO - 10.1111/asj.13091
M3 - Article
VL - 89
SP - 1609
EP - 1621
JO - Animal Science Journal
JF - Animal Science Journal
SN - 1344-3941
IS - 11
ER -