Analysis of extrachromosomal homologous recombination in cultured silkworm cells

Hiroaki Mon, Takahiro Kusakabe, Hisanori Bando, Katsura Kojima, Yutaka Kawaguchi, Katsumi Koga

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

Double-strand breaks (DSBs) are potentially lethal lesions causing the loss of chromosomal information. Eukaryotic cells have evolved the error-free repair systems of DSBs by homologous recombination (HR) through gene conversion with or without crossing over. In this study, we have developed a rapid assay system for extrachromosomal HR events in the cultured silkworm BmN4 cells. When HR occurs within the disrupted luciferase gene, an enzymatically active luciferase is restored and expressed. Our results strongly suggest that error-prone single strand annealing (SSA) accounts for the majority of extrachromosomal recombination processes in the cells. However, upon the substrates which cannot be repaired through SSA, DSBs were efficiently repaired though gene conversion. The rapid and sensitive HR assay system developed in the present study is expected to be a powerful tool for the identification and analysis of HR-related genes in the silkworm.

元の言語英語
ページ(範囲)684-690
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
312
発行部数3
DOI
出版物ステータス出版済み - 12 19 2003

Fingerprint

Gene Conversion
Bombyx
Homologous Recombination
Luciferases
Cultured Cells
Assays
Genes
Annealing
Repair
Substrates
Eukaryotic Cells
Genetic Recombination

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Analysis of extrachromosomal homologous recombination in cultured silkworm cells. / Mon, Hiroaki; Kusakabe, Takahiro; Bando, Hisanori; Kojima, Katsura; Kawaguchi, Yutaka; Koga, Katsumi.

:: Biochemical and Biophysical Research Communications, 巻 312, 番号 3, 19.12.2003, p. 684-690.

研究成果: ジャーナルへの寄稿記事

Mon, Hiroaki ; Kusakabe, Takahiro ; Bando, Hisanori ; Kojima, Katsura ; Kawaguchi, Yutaka ; Koga, Katsumi. / Analysis of extrachromosomal homologous recombination in cultured silkworm cells. :: Biochemical and Biophysical Research Communications. 2003 ; 巻 312, 番号 3. pp. 684-690.
@article{a4b2c07b6d58478a92487882ad01c84d,
title = "Analysis of extrachromosomal homologous recombination in cultured silkworm cells",
abstract = "Double-strand breaks (DSBs) are potentially lethal lesions causing the loss of chromosomal information. Eukaryotic cells have evolved the error-free repair systems of DSBs by homologous recombination (HR) through gene conversion with or without crossing over. In this study, we have developed a rapid assay system for extrachromosomal HR events in the cultured silkworm BmN4 cells. When HR occurs within the disrupted luciferase gene, an enzymatically active luciferase is restored and expressed. Our results strongly suggest that error-prone single strand annealing (SSA) accounts for the majority of extrachromosomal recombination processes in the cells. However, upon the substrates which cannot be repaired through SSA, DSBs were efficiently repaired though gene conversion. The rapid and sensitive HR assay system developed in the present study is expected to be a powerful tool for the identification and analysis of HR-related genes in the silkworm.",
author = "Hiroaki Mon and Takahiro Kusakabe and Hisanori Bando and Katsura Kojima and Yutaka Kawaguchi and Katsumi Koga",
year = "2003",
month = "12",
day = "19",
doi = "10.1016/j.bbrc.2003.10.169",
language = "English",
volume = "312",
pages = "684--690",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Analysis of extrachromosomal homologous recombination in cultured silkworm cells

AU - Mon, Hiroaki

AU - Kusakabe, Takahiro

AU - Bando, Hisanori

AU - Kojima, Katsura

AU - Kawaguchi, Yutaka

AU - Koga, Katsumi

PY - 2003/12/19

Y1 - 2003/12/19

N2 - Double-strand breaks (DSBs) are potentially lethal lesions causing the loss of chromosomal information. Eukaryotic cells have evolved the error-free repair systems of DSBs by homologous recombination (HR) through gene conversion with or without crossing over. In this study, we have developed a rapid assay system for extrachromosomal HR events in the cultured silkworm BmN4 cells. When HR occurs within the disrupted luciferase gene, an enzymatically active luciferase is restored and expressed. Our results strongly suggest that error-prone single strand annealing (SSA) accounts for the majority of extrachromosomal recombination processes in the cells. However, upon the substrates which cannot be repaired through SSA, DSBs were efficiently repaired though gene conversion. The rapid and sensitive HR assay system developed in the present study is expected to be a powerful tool for the identification and analysis of HR-related genes in the silkworm.

AB - Double-strand breaks (DSBs) are potentially lethal lesions causing the loss of chromosomal information. Eukaryotic cells have evolved the error-free repair systems of DSBs by homologous recombination (HR) through gene conversion with or without crossing over. In this study, we have developed a rapid assay system for extrachromosomal HR events in the cultured silkworm BmN4 cells. When HR occurs within the disrupted luciferase gene, an enzymatically active luciferase is restored and expressed. Our results strongly suggest that error-prone single strand annealing (SSA) accounts for the majority of extrachromosomal recombination processes in the cells. However, upon the substrates which cannot be repaired through SSA, DSBs were efficiently repaired though gene conversion. The rapid and sensitive HR assay system developed in the present study is expected to be a powerful tool for the identification and analysis of HR-related genes in the silkworm.

UR - http://www.scopus.com/inward/record.url?scp=0344552852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344552852&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2003.10.169

DO - 10.1016/j.bbrc.2003.10.169

M3 - Article

C2 - 14680819

AN - SCOPUS:0344552852

VL - 312

SP - 684

EP - 690

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -