Analysis of extrachromosomal homologous recombination in cultured silkworm cells

Hiroaki Mon, Takahiro Kusakabe, Hisanori Bando, Katsura Kojima, Yutaka Kawaguchi, Katsumi Koga

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

Double-strand breaks (DSBs) are potentially lethal lesions causing the loss of chromosomal information. Eukaryotic cells have evolved the error-free repair systems of DSBs by homologous recombination (HR) through gene conversion with or without crossing over. In this study, we have developed a rapid assay system for extrachromosomal HR events in the cultured silkworm BmN4 cells. When HR occurs within the disrupted luciferase gene, an enzymatically active luciferase is restored and expressed. Our results strongly suggest that error-prone single strand annealing (SSA) accounts for the majority of extrachromosomal recombination processes in the cells. However, upon the substrates which cannot be repaired through SSA, DSBs were efficiently repaired though gene conversion. The rapid and sensitive HR assay system developed in the present study is expected to be a powerful tool for the identification and analysis of HR-related genes in the silkworm.

本文言語英語
ページ(範囲)684-690
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
312
3
DOI
出版ステータス出版済み - 12 19 2003

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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