Analysis of GPI-AP diffusion on plasma membrane of endothelial cell using photochromic fluorescent protein

Naoto Kawasaki, Tomoya Shimada, Takekazu Fukui, Keita Hamasaki, Susumu Kudo

研究成果: Contribution to journalReview article査読

抄録

The viscosity of the endothelial cell membrane changes during cell migration or in response to shear stress exposure. Interestingly, the degree of viscosity change differs among the various regions of the endothelial cell. These local variations in membrane viscosity may contribute to spatially specific signal transductions. It is unclear, however, whether local membrane viscosity affects to membrane protein dynamics. To address this issue, we examined membrane protein diffusion at different sites in endothelial cells. We fused the photocromic fluorescent protein Dronpa to glycosil phosphatidylinositol-anchored protein (DGGPI) which diffuses on the extracellular surface of the endothelial cell membrane. The DGGPI diffusion coefficient D was 0.165 ± 0.013 μm 2/s (mean ± SE) at 37°C. Examining different sites in the endothelial cells at 28°C (room temperature) revealed that the DGGPI diffusion coefficient at the cell edge was 27% lower than the value measured at the of cell body center.

本文言語英語
ページ(範囲)194-205
ページ数12
ジャーナルNihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
78
785
DOI
出版ステータス出版済み - 2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • 凝縮系物理学
  • 機械工学

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