Analysis of immune reconstitution after autologous CD34 + stem/progenitor cell transplantation for systemic sclerosis: Predominant reconstitution of Th1 CD4 + t cells

Hiroshi Tsukamoto, Koji Nagafuji, Takahiko Horiuchi, Hiroki Mitoma, Hiroaki Niiro, Yojiro Arinobu, Yasushi Inoue, Kentaro To, Toshihiro Miyamoto, Hiromi Iwasaki, Takanori Teshima, Mine Harada, Koichi Akashi

研究成果: Contribution to journalArticle

37 被引用数 (Scopus)

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Objective: The aim of this study is to evaluate the mechanism of long-term effect of autologous haematopoietic stem cell transplantation (ASCT) in treatment of SSc. Methods: Eleven patients (three males and eight females) with SSc were enrolled. Blood mononuclear cells were harvested after mobilization treatment with CYC and G-CSF. CD34 + haematopoietic stem/progenitor cell fractions were purified and cryopreserved. Patients were transplanted with >2 × 10/kg autologous CD34 + cells after high-dose CYC (50 mg/kg for 4 days) conditioning. Immune reconstitution was evaluated serially by analysing lymphocyte subpopulations for 36 months. Results: Progressive improvement of skin sclerosis has been observed for 3 years in most of the patients. The serum level of anti-Scl-70, an auto-antibody specific to SSc, was progressively decreased after ASCT. Improvement of skin sclerosis was significantly associated with the change in the serum anti-Scl-70 level after ASCT at 36 months. Serum levels of KL-6 and surfactant protein D, indicators for interstitial pneumonia activity, were also significantly decreased. The number of CD8+ T cells immediately recovered within a month after ASCT, while the number of CD4+ T cells remained low for >36 months post-transplant. The majority of CD4 + cells were memory but not naïve T cells, and regulatory CD4 + T cells were not recovered. Th1/Th2 ratio was significantly increased after ASCT. Conclusions: ASCT with purified CD34 + cells was effective in controlling the disease activity of SSc. Th1/Th2 ratio was significantly increased for at least 3 years after ASCT.

本文言語英語
論文番号keq414
ページ(範囲)944-952
ページ数9
ジャーナルRheumatology
50
5
DOI
出版ステータス出版済み - 5 2011

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Pharmacology (medical)

フィンガープリント 「Analysis of immune reconstitution after autologous CD34 <sup>+</sup> stem/progenitor cell transplantation for systemic sclerosis: Predominant reconstitution of Th1 CD4 <sup>+</sup> t cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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