TY - JOUR
T1 - Analysis of long-chain polyprenols using supercritical fluid chromatography and matrix-assisted laser desorption ionization time-of-flight mass spectrometry
AU - Bamba, T.
AU - Fukusaki, E.
AU - Nakazawa, Y.
AU - Sato, H.
AU - Ute, K.
AU - Kitayama, T.
AU - Kobayashi, A.
N1 - Funding Information:
This work was supported by the New Energy and Industrial Technology Development Organization (NEDO). Thanks are due to the Venture Business Laboratory, Osaka University for assistance in performing the NMR experiments on a Varian UNITY INOVA NMR 750 spectrometer.
PY - 2003/5/2
Y1 - 2003/5/2
N2 - The separation of long-chain polyprenols was successfully achieved using supercritical fluid chromatography (SFC). Each 100-mer greater component was separated using tetrahydrofuran as a mobile phase modifier. The molecular mass distributions derived from SFC analyses agreed with the results of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses. The number-average molecular mass calculated by MALDI-TOF-MS data were also in accord with the results of quantitative 1H-NMR analysis of terminal groups. A combination of SFC and MALDI-TOF-MS analyses is a powerful tool for the elucidation of the complicated structures of natural polyprenols.
AB - The separation of long-chain polyprenols was successfully achieved using supercritical fluid chromatography (SFC). Each 100-mer greater component was separated using tetrahydrofuran as a mobile phase modifier. The molecular mass distributions derived from SFC analyses agreed with the results of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses. The number-average molecular mass calculated by MALDI-TOF-MS data were also in accord with the results of quantitative 1H-NMR analysis of terminal groups. A combination of SFC and MALDI-TOF-MS analyses is a powerful tool for the elucidation of the complicated structures of natural polyprenols.
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U2 - 10.1016/S0021-9673(03)00498-9
DO - 10.1016/S0021-9673(03)00498-9
M3 - Article
C2 - 12800936
AN - SCOPUS:0038243828
VL - 995
SP - 203
EP - 207
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -