抄録
The separation of long-chain polyprenols was successfully achieved using supercritical fluid chromatography (SFC). Each 100-mer greater component was separated using tetrahydrofuran as a mobile phase modifier. The molecular mass distributions derived from SFC analyses agreed with the results of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses. The number-average molecular mass calculated by MALDI-TOF-MS data were also in accord with the results of quantitative 1H-NMR analysis of terminal groups. A combination of SFC and MALDI-TOF-MS analyses is a powerful tool for the elucidation of the complicated structures of natural polyprenols.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 203-207 |
| ページ数 | 5 |
| ジャーナル | Journal of Chromatography A |
| 巻 | 995 |
| 発行部数 | 1-2 |
| DOI | |
| 出版物ステータス | 出版済み - 5 2 2003 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
これを引用
Analysis of long-chain polyprenols using supercritical fluid chromatography and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. / Bamba, T.; Fukusaki, E.; Nakazawa, Y.; Sato, H.; Ute, K.; Kitayama, T.; Kobayashi, A.
：: Journal of Chromatography A, 巻 995, 番号 1-2, 02.05.2003, p. 203-207.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Analysis of long-chain polyprenols using supercritical fluid chromatography and matrix-assisted laser desorption ionization time-of-flight mass spectrometry
AU - Bamba, T.
AU - Fukusaki, E.
AU - Nakazawa, Y.
AU - Sato, H.
AU - Ute, K.
AU - Kitayama, T.
AU - Kobayashi, A.
PY - 2003/5/2
Y1 - 2003/5/2
N2 - The separation of long-chain polyprenols was successfully achieved using supercritical fluid chromatography (SFC). Each 100-mer greater component was separated using tetrahydrofuran as a mobile phase modifier. The molecular mass distributions derived from SFC analyses agreed with the results of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses. The number-average molecular mass calculated by MALDI-TOF-MS data were also in accord with the results of quantitative 1H-NMR analysis of terminal groups. A combination of SFC and MALDI-TOF-MS analyses is a powerful tool for the elucidation of the complicated structures of natural polyprenols.
AB - The separation of long-chain polyprenols was successfully achieved using supercritical fluid chromatography (SFC). Each 100-mer greater component was separated using tetrahydrofuran as a mobile phase modifier. The molecular mass distributions derived from SFC analyses agreed with the results of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses. The number-average molecular mass calculated by MALDI-TOF-MS data were also in accord with the results of quantitative 1H-NMR analysis of terminal groups. A combination of SFC and MALDI-TOF-MS analyses is a powerful tool for the elucidation of the complicated structures of natural polyprenols.
UR - http://www.scopus.com/inward/record.url?scp=0038243828&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038243828&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(03)00498-9
DO - 10.1016/S0021-9673(03)00498-9
M3 - Article
C2 - 12800936
AN - SCOPUS:0038243828
VL - 995
SP - 203
EP - 207
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -