Analysis of long-chain polyprenols using supercritical fluid chromatography and matrix-assisted laser desorption ionization time-of-flight mass spectrometry

T. Bamba, E. Fukusaki, Y. Nakazawa, H. Sato, K. Ute, T. Kitayama, A. Kobayashi

研究成果: ジャーナルへの寄稿学術誌査読

25 被引用数 (Scopus)

抄録

The separation of long-chain polyprenols was successfully achieved using supercritical fluid chromatography (SFC). Each 100-mer greater component was separated using tetrahydrofuran as a mobile phase modifier. The molecular mass distributions derived from SFC analyses agreed with the results of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses. The number-average molecular mass calculated by MALDI-TOF-MS data were also in accord with the results of quantitative 1H-NMR analysis of terminal groups. A combination of SFC and MALDI-TOF-MS analyses is a powerful tool for the elucidation of the complicated structures of natural polyprenols.

本文言語英語
ページ(範囲)203-207
ページ数5
ジャーナルJournal of Chromatography A
995
1-2
DOI
出版ステータス出版済み - 5月 2 2003
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生化学
  • 有機化学

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