Analysis of membrane topology of neutral sphingomyelinase 2

Motohiro Tani, Yusuf A. Hannun

研究成果: Contribution to journalArticle査読

49 被引用数 (Scopus)

抄録

Neutral sphingomyelinase 2 (nSMase2), which has two hydrophobic segments at its NH2-terminus, plays an important role in ceramide-mediated cell regulation. Here, we investigated the membrane topology of nSMase2. When a double-tagged nSMase2 at both the NH2 and COOH termini, was overexpressed in MCF-7 cells, the signals from both tags were detected in the inner leaflet of the plasma membrane. Furthermore, insertion of a tag into the internal sequence and green fluorescent protein-fused deletion mutants revealed that the entire catalytic region of the protein was located on the cytosolic face of the membranes and each hydrophobic segment is integrated into the membranes, but unlikely to span the entire membrane. These results indicate the presence of the enzyme in the inner leaflet of plasma membrane.

本文言語英語
ページ(範囲)1323-1328
ページ数6
ジャーナルFEBS Letters
581
7
DOI
出版ステータス出版済み - 4 3 2007
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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