Analysis of ornithine decarboxylase messenger ribonucleic acid expression in colorectal carcinoma

Koshi Mimori, Masaki Mori, Takeshi Shiraishi, Tatsuo Fujie, Kinya Baba, Hiroki Kusumoto, Masaru Haraguchi, Hiroaki Ueo, Tsuyoshi Akiyoshi

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

PURPOSE: Ornithine decarboxylase (ODC) is a rate-limiting enzyme for polyamine synthesis. An elevated protein level of ODC was observed in the tumors. There has been, however, little information reported so far on the expression of ODC messenger ribonucleic acid (mRNA) in clinical colorectal carcinomas. In vitro studies disclosed that the transcriptions of the ODC gene is regulated by the c-myc gene. METHODS: The expression of ODC and c- myc mRNA in biopsy specimens obtained from both tumor tissue and the corresponding normal tissue was examined by the reverse transcriptase polymerase chain reaction method in 40 cases of colorectal carcinoma. RESULTS: The expression of ODC mRNA was observed in both tumor tissue and normal tissue. The tumor to normal ratio of ODC mRNA was higher in cases with deeply invasive tumors than in cases with shallow tumors, and it was also higher in Dukes B or C cases than in Dukes A cases. There was a significant correlation between the tumor to normal ratio of c-myc mRNA and that of ODC mRNA in each case. CONCLUSIONS: These findings suggested that 1) the study of the expression of ODC mRNA may be useful for preoperatively predicting more advanced disease of colon carcinoma, and 2) there was a significant correlation between expression of ODC and c-myc mRNA in the clinical samples, which was similar to the findings of a previous in vitro study.

元の言語英語
ページ(範囲)1095-1100
ページ数6
ジャーナルDiseases of the colon and rectum
40
発行部数9
DOI
出版物ステータス出版済み - 9 1 1997

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Ornithine Decarboxylase
Colorectal Neoplasms
RNA
Neoplasms
myc Genes
Polyamines
Reverse Transcriptase Polymerase Chain Reaction
Colon
Carcinoma
Biopsy

All Science Journal Classification (ASJC) codes

  • Gastroenterology

これを引用

Analysis of ornithine decarboxylase messenger ribonucleic acid expression in colorectal carcinoma. / Mimori, Koshi; Mori, Masaki; Shiraishi, Takeshi; Fujie, Tatsuo; Baba, Kinya; Kusumoto, Hiroki; Haraguchi, Masaru; Ueo, Hiroaki; Akiyoshi, Tsuyoshi.

:: Diseases of the colon and rectum, 巻 40, 番号 9, 01.09.1997, p. 1095-1100.

研究成果: ジャーナルへの寄稿記事

Mimori, K, Mori, M, Shiraishi, T, Fujie, T, Baba, K, Kusumoto, H, Haraguchi, M, Ueo, H & Akiyoshi, T 1997, 'Analysis of ornithine decarboxylase messenger ribonucleic acid expression in colorectal carcinoma', Diseases of the colon and rectum, 巻. 40, 番号 9, pp. 1095-1100. https://doi.org/10.1007/BF02050936
Mimori, Koshi ; Mori, Masaki ; Shiraishi, Takeshi ; Fujie, Tatsuo ; Baba, Kinya ; Kusumoto, Hiroki ; Haraguchi, Masaru ; Ueo, Hiroaki ; Akiyoshi, Tsuyoshi. / Analysis of ornithine decarboxylase messenger ribonucleic acid expression in colorectal carcinoma. :: Diseases of the colon and rectum. 1997 ; 巻 40, 番号 9. pp. 1095-1100.
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T1 - Analysis of ornithine decarboxylase messenger ribonucleic acid expression in colorectal carcinoma

AU - Mimori, Koshi

AU - Mori, Masaki

AU - Shiraishi, Takeshi

AU - Fujie, Tatsuo

AU - Baba, Kinya

AU - Kusumoto, Hiroki

AU - Haraguchi, Masaru

AU - Ueo, Hiroaki

AU - Akiyoshi, Tsuyoshi

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N2 - PURPOSE: Ornithine decarboxylase (ODC) is a rate-limiting enzyme for polyamine synthesis. An elevated protein level of ODC was observed in the tumors. There has been, however, little information reported so far on the expression of ODC messenger ribonucleic acid (mRNA) in clinical colorectal carcinomas. In vitro studies disclosed that the transcriptions of the ODC gene is regulated by the c-myc gene. METHODS: The expression of ODC and c- myc mRNA in biopsy specimens obtained from both tumor tissue and the corresponding normal tissue was examined by the reverse transcriptase polymerase chain reaction method in 40 cases of colorectal carcinoma. RESULTS: The expression of ODC mRNA was observed in both tumor tissue and normal tissue. The tumor to normal ratio of ODC mRNA was higher in cases with deeply invasive tumors than in cases with shallow tumors, and it was also higher in Dukes B or C cases than in Dukes A cases. There was a significant correlation between the tumor to normal ratio of c-myc mRNA and that of ODC mRNA in each case. CONCLUSIONS: These findings suggested that 1) the study of the expression of ODC mRNA may be useful for preoperatively predicting more advanced disease of colon carcinoma, and 2) there was a significant correlation between expression of ODC and c-myc mRNA in the clinical samples, which was similar to the findings of a previous in vitro study.

AB - PURPOSE: Ornithine decarboxylase (ODC) is a rate-limiting enzyme for polyamine synthesis. An elevated protein level of ODC was observed in the tumors. There has been, however, little information reported so far on the expression of ODC messenger ribonucleic acid (mRNA) in clinical colorectal carcinomas. In vitro studies disclosed that the transcriptions of the ODC gene is regulated by the c-myc gene. METHODS: The expression of ODC and c- myc mRNA in biopsy specimens obtained from both tumor tissue and the corresponding normal tissue was examined by the reverse transcriptase polymerase chain reaction method in 40 cases of colorectal carcinoma. RESULTS: The expression of ODC mRNA was observed in both tumor tissue and normal tissue. The tumor to normal ratio of ODC mRNA was higher in cases with deeply invasive tumors than in cases with shallow tumors, and it was also higher in Dukes B or C cases than in Dukes A cases. There was a significant correlation between the tumor to normal ratio of c-myc mRNA and that of ODC mRNA in each case. CONCLUSIONS: These findings suggested that 1) the study of the expression of ODC mRNA may be useful for preoperatively predicting more advanced disease of colon carcinoma, and 2) there was a significant correlation between expression of ODC and c-myc mRNA in the clinical samples, which was similar to the findings of a previous in vitro study.

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