Analysis of protein dynamics during cytokinesis in budding yeast

S. Okada, C. Wloka, E. Bi

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

Cytokinesis is essential for development and survival of all organisms by increasing cell number and diversity. It is a highly regulated process that requires spatiotemporal coordination of hundreds of proteins functioning in the assembly, constriction, and disassembly of a contractile actomyosin ring, targeted vesicle fusion, and localized extracellular matrix remodeling. Cytokinesis has been studied in multiple systems with a wide range of technologies to learn the common principles. In this chapter, we describe the analysis of protein dynamics during cytokinesis in the budding yeast Saccharomyces cerevisiae by several live-cell imaging methods. This, in combination with the power of yeast genetics, has yielded novel insights into the mechanism of cytokinesis. Similar approaches are increasingly used to study this fundamental process in more complex systems.

本文言語英語
ページ(範囲)25-45
ページ数21
ジャーナルMethods in Cell Biology
137
DOI
出版ステータス出版済み - 12 1 2017

All Science Journal Classification (ASJC) codes

  • Cell Biology

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