Analysis of Small RNA Profiles During Development

Toshiaki Watanabe, Yasushi Totoki, Hiroyuki Sasaki, Naojiro Minami, Hiroshi Imai

研究成果: Chapter in Book/Report/Conference proceedingChapter

10 被引用数 (Scopus)

抄録

Small RNAs ranging in size between 20 and 32 nt regulate gene expression through chromatin modification, mRNA degradation, and translational repression. Three major classes of small RNAs have been characterized: microRNAs (miRNAs), short interfering RNAs (siRNAs), and Piwi-interacting RNAs (piRNAs). miRNAs are expressed in a developmentally regulated and tissue-specific manner and are involved in development and cell differentiation. siRNAs are mainly involved in defense against transposons and viruses. piRNAs are expressed in germ cells and stem cells and are thought to repress transposition of retrotransposons. In this chapter, we describe the methods of small RNA cloning, annotation and classification, and their expression analysis during development.

本文言語英語
ホスト出版物のタイトルMicroRNA Methods
編集者John Rossi, Gregory Hannon
ページ155-169
ページ数15
DOI
出版ステータス出版済み - 2007
外部発表はい

出版物シリーズ

名前Methods in Enzymology
427
ISSN(印刷版)0076-6879

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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