We describe a novel method to investigate variability of T cell responses of PBLs using large libraries of synthetic pepiides. Motif aided peptide epitope library for DRB 1*0405 restricted T cell response was synthesized, containing fixed amino acids representing ihe major histocompatibility complex (MHC) class II anchor residues for HLA-DRB1*0405 (YXXLXAXXD). We synthesized 135 sets of peptide libraries, each of which contains 729 peptide species. T cell responses of PBLs were examined for two HLA-DR identical donors (DRB1*0405/1502) and one non identical donors (DRB 1*0406/0901). Appreciable T cell responses were observed in both donors. The response pattern of PBLs against two HLA-DR identical donors were similar while that between HLA-DR non identical donors were different, suggesting the strong HLA restriction of T cell response. The library will be useful tool to investigate the pathogenic T cell response such as auto immune diseases.
|ジャーナル||Japanese Journal of Human Genetics|
|出版ステータス||出版済み - 12 1 1996|
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