Analysis of the human adrenodoxin promoter: Evidence for its activity

Bon chu Chung, Char Chang Lai, Cheng Hui Lin, Chi Yao Chang

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

Adrenodoxin is an iron-sulfur protein serving as an electron transfer intermediate in the mitochondrial cytochrome P450 system. To study its transcriptional regulation we construct a human adrenodoxin genomic clone which includes 333 bp of DNA upstream of the mRNA start site. This DNA contains a TATA box and two GC boxes. When we place it in front of the CAT reporter gene and transfect it into the recipient cell lines, it directs transcription of the CAT gene. This DNA in reverse orientation does not show any transcriptional activity. This promoter element functions in three mammalian cell lines: JEG-3, COS-1, and Y-1.

本文言語英語
ページ(範囲)343-348
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
159
1
DOI
出版ステータス出版済み - 2 28 1989

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Analysis of the human adrenodoxin promoter: Evidence for its activity」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル