Analysis of the in vitro translation product of a novel-type Drosophila melanogaster aldolase mRNA in which two carboxyl-terminal exons remain unspliced

Yasushi Sugimoto, Takahiro Kusakabe, Tatsuo Kai, Tsuyoshi Okamura, Katsumi Koga, Katsuji Hori

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

Drosophila melanogaster generates three different types of aldolase mRNAs from a single gene by selective usage of the triplicate exons 4 (4α, 4β, and 4γ), which encode three different isozymes having respective carboxyl termini. We have found the presence of a novel-type mRNA (named αβ) in which two final exons, 4α and 4β, were retained unspliced. Herein, a cDNA clone containing the αβ) sequence was inserted into pINIII and expressed in an Escherichia coli system. The product, which exhibited aldolase activity, was found to be isozyme α from the primary structure and the enzymological properties, with the 4α sequence alone being present as the carboxyl terminus. In tissues of D. melanogaster, the production of mRNA encoding exon 4α is known to be restrained to a low level. This may be understood by the fact that the aldolase gene of this species does not have a typical poly(A) signal at the 3′ end in exon 4α. Instead, the transcript encoding exons, 4α and 4β, might be produced when AATATA, which resides downstream of the coding frame in exon 4β, is recognized as a poly(A) signal during RNA processing.

本文言語英語
ページ(範囲)361-366
ページ数6
ジャーナルArchives of Biochemistry and Biophysics
323
2
DOI
出版ステータス出版済み - 11 1995
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学

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