Analysis of the light regulatory mechanism in carotenoid production in Rhodosporidium toruloides NBRC 10032

Khanh Dung Pham, Yuuki Hakozaki, Takeru Takamizawa, Atsushi Yamazaki, Harutake Yamazaki, Kazuki Mori, Sachiyo Aburatani, Kosuke Tashiro, Satoru Kuhara, Hiroaki Takaku, Yosuke Shida, Wataru Ogasawara

研究成果: Contribution to journalArticle査読

抄録

Light stimulates carotenoid production in an oleaginous yeast Rhodosporidium toruloides NBRC 10032 by promoting carotenoid biosynthesis genes. These genes undergo two-step transcriptional activation. The potential light regulator, Cryptochrome DASH (CRY1), has been suggested to contribute to this mechanism. In this study, based on KU70 (a component of nonhomologous end joining (NHEJ)) disrupting background, CRY1 disruptant was constructed to clarify CRY1 function. From analysis of CRY1 disruptant, it was suggested that CRY1 has the activation role of the carotenogenic gene expression. To obtain further insights into the light response, mutants varying carotenoid production were generated. Through analysis of mutants, the existence of the control two-step gene activation was proposed. In addition, our data analysis showed the strong possibility that R. toruloides NBRC 10032 is a homo-diploid strain.

本文言語英語
ページ(範囲)1899-1909
ページ数11
ジャーナルBioscience, Biotechnology and Biochemistry
85
8
DOI
出版ステータス出版済み - 8 1 2021

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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