Analysis of the Molecular Recognition of L-lsoleucine by Isoleucyl-tRNA Synthetase

Shigeyuki Yokoyama, Daisuke Kohda, Tatsuo Miyazawa

研究成果: Contribution to journalArticle査読

抄録

The 400-MHz 1H-NMR spectra of L-isoleucine were measured in the presence of Escherichia coli isoleucyl-tRNA synthetase (IleRS). Because of chemical exchange of L-isoleucine between the free state and the IleRS-bound state, transferred nuclear Overhauser effect (TRNOE) was observed among proton resonances of L-isoleucine. The IleRS-bound L-isoleucine was found to take the gauche+ form about the Cα-Cβ bond and the trans form about the Cβ-Cγ1 bond. The TRNOE analysis is useful for studying the amino acid discrimination mechanism of ammoacyl-tRNA synthetases.

本文言語英語
ページ(範囲)402-404
ページ数3
ジャーナルNIPPON KAGAKU KAISHI
1987
3
DOI
出版ステータス出版済み - 1 1 1987
外部発表はい

All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 化学工学(全般)

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