Anatomical distribution and immunological characteristics of epidermolysis bullosa acquisita antigen and bullous pemphigoid antigen

Masutaka Furue, M. IWATA, K. TAMAKI, Y. ISHIBASHI

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

A Japanese patient with epidermolysis bullosa acquisita (EBA) was autopsied, and direct immunofluorescence (DIF) testing was performed. Using this patient's serum (EBA serum) and three bullous pemphigoid (BP) sera, the anatomical distribution and immunological character‐istics of EBA antigen and BP antigen were investigated by indirect immunofluorescence (IIF). EBA antigen showed the same anatomical distribution as BP antigen in DIF and IIF studies; both antigens were limited to the skin, tongue, oesophagus, trachea, cornea and bladder. EBA antigen was located on the dermal side of both NaCI and PBS‐separated skin, whereas BP antigen was limited to the epidermal side. Ethanol fixation abrogated the antigenic stability of BP antigen, but not that of HBA antigen. No difference was found when acetone or formalin fixation was used. The separation methods and prefixation in ethanol could be useful techniques applicable to the classification of the bullous disorders which manifest circulating anti‐BMZ antibodies.

元の言語英語
ページ(範囲)651-659
ページ数9
ジャーナルBritish Journal of Dermatology
114
発行部数6
DOI
出版物ステータス出版済み - 1 1 1986
外部発表Yes

Fingerprint

Bullous Pemphigoid
Antigens
Epidermolysis Bullosa Acquisita
Direct Fluorescent Antibody Technique
Indirect Fluorescent Antibody Technique
Skin
Ethanol
Serum
Acetone
Trachea
Tongue
Cornea
Formaldehyde
Esophagus
epidermolysis bullosa acquisita antigen
Urinary Bladder
Antibodies

All Science Journal Classification (ASJC) codes

  • Dermatology

これを引用

Anatomical distribution and immunological characteristics of epidermolysis bullosa acquisita antigen and bullous pemphigoid antigen. / Furue, Masutaka; IWATA, M.; TAMAKI, K.; ISHIBASHI, Y.

:: British Journal of Dermatology, 巻 114, 番号 6, 01.01.1986, p. 651-659.

研究成果: ジャーナルへの寄稿記事

@article{688599212f8b42c2819d512d2d56bbdc,
title = "Anatomical distribution and immunological characteristics of epidermolysis bullosa acquisita antigen and bullous pemphigoid antigen",
abstract = "A Japanese patient with epidermolysis bullosa acquisita (EBA) was autopsied, and direct immunofluorescence (DIF) testing was performed. Using this patient's serum (EBA serum) and three bullous pemphigoid (BP) sera, the anatomical distribution and immunological character‐istics of EBA antigen and BP antigen were investigated by indirect immunofluorescence (IIF). EBA antigen showed the same anatomical distribution as BP antigen in DIF and IIF studies; both antigens were limited to the skin, tongue, oesophagus, trachea, cornea and bladder. EBA antigen was located on the dermal side of both NaCI and PBS‐separated skin, whereas BP antigen was limited to the epidermal side. Ethanol fixation abrogated the antigenic stability of BP antigen, but not that of HBA antigen. No difference was found when acetone or formalin fixation was used. The separation methods and prefixation in ethanol could be useful techniques applicable to the classification of the bullous disorders which manifest circulating anti‐BMZ antibodies.",
author = "Masutaka Furue and M. IWATA and K. TAMAKI and Y. ISHIBASHI",
year = "1986",
month = "1",
day = "1",
doi = "10.1111/j.1365-2133.1986.tb04872.x",
language = "English",
volume = "114",
pages = "651--659",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Anatomical distribution and immunological characteristics of epidermolysis bullosa acquisita antigen and bullous pemphigoid antigen

AU - Furue, Masutaka

AU - IWATA, M.

AU - TAMAKI, K.

AU - ISHIBASHI, Y.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - A Japanese patient with epidermolysis bullosa acquisita (EBA) was autopsied, and direct immunofluorescence (DIF) testing was performed. Using this patient's serum (EBA serum) and three bullous pemphigoid (BP) sera, the anatomical distribution and immunological character‐istics of EBA antigen and BP antigen were investigated by indirect immunofluorescence (IIF). EBA antigen showed the same anatomical distribution as BP antigen in DIF and IIF studies; both antigens were limited to the skin, tongue, oesophagus, trachea, cornea and bladder. EBA antigen was located on the dermal side of both NaCI and PBS‐separated skin, whereas BP antigen was limited to the epidermal side. Ethanol fixation abrogated the antigenic stability of BP antigen, but not that of HBA antigen. No difference was found when acetone or formalin fixation was used. The separation methods and prefixation in ethanol could be useful techniques applicable to the classification of the bullous disorders which manifest circulating anti‐BMZ antibodies.

AB - A Japanese patient with epidermolysis bullosa acquisita (EBA) was autopsied, and direct immunofluorescence (DIF) testing was performed. Using this patient's serum (EBA serum) and three bullous pemphigoid (BP) sera, the anatomical distribution and immunological character‐istics of EBA antigen and BP antigen were investigated by indirect immunofluorescence (IIF). EBA antigen showed the same anatomical distribution as BP antigen in DIF and IIF studies; both antigens were limited to the skin, tongue, oesophagus, trachea, cornea and bladder. EBA antigen was located on the dermal side of both NaCI and PBS‐separated skin, whereas BP antigen was limited to the epidermal side. Ethanol fixation abrogated the antigenic stability of BP antigen, but not that of HBA antigen. No difference was found when acetone or formalin fixation was used. The separation methods and prefixation in ethanol could be useful techniques applicable to the classification of the bullous disorders which manifest circulating anti‐BMZ antibodies.

UR - http://www.scopus.com/inward/record.url?scp=0022515865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022515865&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2133.1986.tb04872.x

DO - 10.1111/j.1365-2133.1986.tb04872.x

M3 - Article

C2 - 3521710

AN - SCOPUS:0022515865

VL - 114

SP - 651

EP - 659

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 6

ER -