Anchoring of the 26S proteasome to the organellar membrane by FKBP38

Tadashi Nakagawa, Michiko Shirane, Shun Ichiro Lemura, Tohru Natsume, Keiichi Nakayama

研究成果: ジャーナルへの寄稿記事

39 引用 (Scopus)

抄録

FK506-binding protein 38 (FKBP38) is a member of the immunophilin family that resides in the mitochondrial outer membrane and the endoplasmic reticulum (ER) membrane. To investigate the physiological function of FKBP38, we performed a comprehensive search for proteins with which it interacts in human cells by liquid chromatographic and mass spectrometric analysis of FKBP38 immunoprecipitates. Almost all subunits of the 26S proteasome were thus found to interact with FKBP38. In vivo co-immunoprecipitation analyses confirmed that FKBP38 indeed associates with the 26S proteasome via its three tandem tetratricopeptide repeats (TPRs). Binding assays in vitro also revealed that FKBP38 directly interacts with the S4 subunit of the 19S proteasome. Immunofluorescence analysis demonstrated that the subcellular distributions of FKBP38 and the 26S proteasome partially overlapped at mitochondria. Both the abundance and activity of the proteasome in a membrane fraction were markedly reduced for mouse embryonic fibroblasts prepared from Fkbp38-/- mice compared with those prepared from wild-type mice. These results suggest that FKBP38 functions to anchor the 26S proteasome at the organellar membrane.

元の言語英語
ページ(範囲)709-719
ページ数11
ジャーナルGenes to Cells
12
発行部数6
DOI
出版物ステータス出版済み - 6 1 2007

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Tacrolimus Binding Proteins
Membranes
Immunophilins
Tandem Repeat Sequences
ATP dependent 26S protease
Mitochondrial Membranes
Proteasome Endopeptidase Complex
Immunoprecipitation
Endoplasmic Reticulum
Fluorescent Antibody Technique
Mitochondria
Fibroblasts

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

これを引用

Anchoring of the 26S proteasome to the organellar membrane by FKBP38. / Nakagawa, Tadashi; Shirane, Michiko; Lemura, Shun Ichiro; Natsume, Tohru; Nakayama, Keiichi.

:: Genes to Cells, 巻 12, 番号 6, 01.06.2007, p. 709-719.

研究成果: ジャーナルへの寄稿記事

Nakagawa, Tadashi ; Shirane, Michiko ; Lemura, Shun Ichiro ; Natsume, Tohru ; Nakayama, Keiichi. / Anchoring of the 26S proteasome to the organellar membrane by FKBP38. :: Genes to Cells. 2007 ; 巻 12, 番号 6. pp. 709-719.
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AU - Nakayama, Keiichi

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