Androgen regulates Mafb expression through its 3′UTR during mouse urethral masculinization

Shoko Matsushita, Kentaro Suzuki, Yukiko Ogino, Shinjiro Hino, Tetsuya Sato, Mikita Suyama, Takahiro Matsumoto, Akiko Omori, Satoshi Inoue, Gen Yamada

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

External genitalia are prominent organs showing hormone-dependent sexual differentiation. Androgen is an essential regulator of masculinization of the genital tubercle, which is the anlage of external genitalia. We have previously shown that v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB) is an androgen-inducible regulator of embryonic urethral masculinization in mice. However, it remains unclear how androgen regulates Mafb expression. The current study suggests that the Mafb 3′ untranslated region (UTR) is an essential region for its regulation by androgen. We identified 2 functional androgen response elements (AREs) in Mafb 3′UTR. Androgen receptor is bound to such AREs in 3′UTR during urethral masculinization. In addition to 3′UTR, Mafb 5′UTR also showed androgen responsiveness. Moreover, we also demonstrated that β-catenin, one of genital tubercle masculinization factors, may be an additional regulator of Mafb expression during urethral masculinization. This study provides insights to elucidate mechanisms of gene regulation through AREs present in Mafb 3′UTR for a better understanding of the processes of urethral masculinization.

元の言語英語
ページ(範囲)844-857
ページ数14
ジャーナルEndocrinology
157
発行部数2
DOI
出版物ステータス出版済み - 2 2016

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Androgens
Response Elements
Genitalia
Catenins
Sex Differentiation
Fibrosarcoma
Androgen Receptors
3' Untranslated Regions
Oncogenes
Hormones
Genes

All Science Journal Classification (ASJC) codes

  • Endocrinology

これを引用

Androgen regulates Mafb expression through its 3′UTR during mouse urethral masculinization. / Matsushita, Shoko; Suzuki, Kentaro; Ogino, Yukiko; Hino, Shinjiro; Sato, Tetsuya; Suyama, Mikita; Matsumoto, Takahiro; Omori, Akiko; Inoue, Satoshi; Yamada, Gen.

:: Endocrinology, 巻 157, 番号 2, 02.2016, p. 844-857.

研究成果: ジャーナルへの寄稿記事

Matsushita, S, Suzuki, K, Ogino, Y, Hino, S, Sato, T, Suyama, M, Matsumoto, T, Omori, A, Inoue, S & Yamada, G 2016, 'Androgen regulates Mafb expression through its 3′UTR during mouse urethral masculinization', Endocrinology, 巻. 157, 番号 2, pp. 844-857. https://doi.org/10.1210/en.2015-1586
Matsushita, Shoko ; Suzuki, Kentaro ; Ogino, Yukiko ; Hino, Shinjiro ; Sato, Tetsuya ; Suyama, Mikita ; Matsumoto, Takahiro ; Omori, Akiko ; Inoue, Satoshi ; Yamada, Gen. / Androgen regulates Mafb expression through its 3′UTR during mouse urethral masculinization. :: Endocrinology. 2016 ; 巻 157, 番号 2. pp. 844-857.
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AU - Sato, Tetsuya

AU - Suyama, Mikita

AU - Matsumoto, Takahiro

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AU - Inoue, Satoshi

AU - Yamada, Gen

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