Aneuploid plants derived from crosses with triploid grapes through immature seed culture and subsequent embryo culture

S. M. Park, Michikazu Hiramatsu, Akira Wakana

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

Through immature seed culture and subsequent embryo culture, aneuploid plants were derived from various crosses among 184 different triploid hybrid grape vines. In self-pollinations of the 184 vines, 0 to 1.6% of flowers produced immature seeds. In 16 reciprocal crosses between diploid and triploid and between tetraploid and triploid grapes, 0 to 23.0% of flowers produced immature seeds. The immature seeds excised 30-50 days after pollination were cultured for three months on Nitsch and Nitsch medium supplemented with L-glutamine, L-serine, L-cysteine and casein hydrolysate. Embryos developed within the cultured immature seeds were subcultured onto germination medium consisting of MS medium with 1 μM BA. Thirty-four of 137 embryos from 458 immature seeds germinated. Five of the 34 embryos grew normally. The five recovered plants were aneuploids with chromosome numbers from 51 to 59. The rates of embryo and plant recovery were different in different crosses with triploid grapes.

元の言語英語
ページ(範囲)125-133
ページ数9
ジャーナルPlant Cell, Tissue and Organ Culture
59
発行部数2
DOI
出版物ステータス出版済み - 12 1 1999

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embryo culture
aneuploidy
triploidy
grapes
immatures
embryo (plant)
seeds
vines
flowers
casein hydrolysates
reciprocal crosses
self-pollination
glutamine
serine
chromosome number
tetraploidy
cysteine
pollination
diploidy
germination

All Science Journal Classification (ASJC) codes

  • Horticulture

これを引用

Aneuploid plants derived from crosses with triploid grapes through immature seed culture and subsequent embryo culture. / Park, S. M.; Hiramatsu, Michikazu; Wakana, Akira.

:: Plant Cell, Tissue and Organ Culture, 巻 59, 番号 2, 01.12.1999, p. 125-133.

研究成果: ジャーナルへの寄稿記事

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