Anisotropic Incorporation of Lipid-Anchored Myoglobin into a Phospholipid Bilayer Membrane

Itaru Hamachi, Kentaro Nakamura, Akio Fujita, Toyoki Kunitake

研究成果: Contribution to journalArticle査読

30 被引用数 (Scopus)

抄録

Directionally controlled anchoring of myoglobin, a water-soluble globular protein, on a dipalmitoylphos-phatidylcholine (DPPC) bilayer has been successfully conducted. Lipid-anchored myoglobin was obtained from a monoalkylated heme derivative and apomyoglobin. It was indicated by gel filtration and ultrafiltration studies that the lipid-anchored myoglobin Mb(1a), but not native myoglobin and Mb(2a), is bound to the DPPC bilayer membrane in aqueous dispersion. A cast film of the phospholipid and the anchored myoglobin displayed anisotropic ESR signals, which depend on the disposition of the cast film in the magnetic field. These results suggest that myoglobin molecules are placed on the lipid bilayer in a fixed orientation by inserting the anchor alkyl chain into the bilayer. The ESR anisotropy was not observed without the anchor.

本文言語英語
ページ(範囲)4966-4970
ページ数5
ジャーナルJournal of the American Chemical Society
115
12
DOI
出版ステータス出版済み - 6 1 1993

All Science Journal Classification (ASJC) codes

  • 触媒
  • 化学 (全般)
  • 生化学
  • コロイド化学および表面化学

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