抄録
Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R4 different from the expected size-dependence of R3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.
本文言語 | 英語 |
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論文番号 | 7364 |
ジャーナル | Scientific reports |
巻 | 8 |
号 | 1 |
DOI | |
出版ステータス | 出版済み - 12月 1 2018 |
!!!All Science Journal Classification (ASJC) codes
- 一般