Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

Ryota Sakamoto, Vincent Noireaux, Yusuke T. Maeda

研究成果: ジャーナルへの寄稿学術誌査読

23 被引用数 (Scopus)

抄録

Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R4 different from the expected size-dependence of R3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.

本文言語英語
論文番号7364
ジャーナルScientific reports
8
1
DOI
出版ステータス出版済み - 12月 1 2018

!!!All Science Journal Classification (ASJC) codes

  • 一般

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