Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility

Yoshitaka Kanaoka, Daichi Kuniyoshi, Eri Inada, Yohei Koide, Yoshihiro Okamoto, Hideshi Yasui, Yuji Kishima

研究成果: ジャーナルへの寄稿記事

抄録

Background: To investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete sterility owing to a dozen of hybrid sterility loci, termed HS loci, but this complicated genetic system remains poorly understood. Results: Microspores from these interspecific hybrids form sterile pollen but are viable at the immature stage. Application of the anther culture (AC) method caused these immature microspores to induce callus. The segregation distortion of 11 among 13 known HS loci was assessed in the callus population. Using many individual calli, fine mapping of the HS loci was attempted based on heterozygotes produced from chromosome segment substitution lines (CSSLs). Transmission ratio distortion (TRD) from microspores was detected at 6 of 11 HS loci in the callus population. The fine mapping of S 1 and S 19 loci using CSSLs revealed precise distances of markers from the positions of HS loci exhibiting excessive TRD. Conclusions: We demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple HS loci and dramatically shortened the process for mapping hybrid sterility genes.

元の言語英語
記事番号102
ジャーナルPlant Methods
14
発行部数1
DOI
出版物ステータス出版済み - 11 17 2018

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anther culture
Bony Callus
microspores
Infertility
rice
callus
loci
Genes
HS 6
genes
Oryza glaberrima
substitution lines
Population
immatures
Chromosomes
Genetic Loci
Heterozygote
Pollen
chromosomes
segregation distortion

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Genetics
  • Plant Science

これを引用

Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility. / Kanaoka, Yoshitaka; Kuniyoshi, Daichi; Inada, Eri; Koide, Yohei; Okamoto, Yoshihiro; Yasui, Hideshi; Kishima, Yuji.

:: Plant Methods, 巻 14, 番号 1, 102, 17.11.2018.

研究成果: ジャーナルへの寄稿記事

Kanaoka, Yoshitaka ; Kuniyoshi, Daichi ; Inada, Eri ; Koide, Yohei ; Okamoto, Yoshihiro ; Yasui, Hideshi ; Kishima, Yuji. / Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility. :: Plant Methods. 2018 ; 巻 14, 番号 1.
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abstract = "Background: To investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete sterility owing to a dozen of hybrid sterility loci, termed HS loci, but this complicated genetic system remains poorly understood. Results: Microspores from these interspecific hybrids form sterile pollen but are viable at the immature stage. Application of the anther culture (AC) method caused these immature microspores to induce callus. The segregation distortion of 11 among 13 known HS loci was assessed in the callus population. Using many individual calli, fine mapping of the HS loci was attempted based on heterozygotes produced from chromosome segment substitution lines (CSSLs). Transmission ratio distortion (TRD) from microspores was detected at 6 of 11 HS loci in the callus population. The fine mapping of S 1 and S 19 loci using CSSLs revealed precise distances of markers from the positions of HS loci exhibiting excessive TRD. Conclusions: We demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple HS loci and dramatically shortened the process for mapping hybrid sterility genes.",
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AU - Koide, Yohei

AU - Okamoto, Yoshihiro

AU - Yasui, Hideshi

AU - Kishima, Yuji

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