Anti-allergic activity of polyphenolic compounds isolated from olive mill wastes

Rogers Mwakalukwa, Ahmed Ashour, Yhiya Amen, Yasuharu Niwa, Sonam Tamrakar, Tomofumi Miyamoto, Kuniyoshi Shimizu

研究成果: ジャーナルへの寄稿記事

抄録

We report the isolation and anti-allergic activity (degranulation reduction)of six polyphenolic compounds, one of them being novel, from olive mill wastes (OMW). Additionally, we evaluated their ability to reduce; intracellular Ca 2+ levels and expression of calcium channel proteins in RBL-2H3 cells, to assist the possible characterization of the mechanisms involved. The novel compound, new hemialdehydic decarboxymethylated oleuropein aglycone, HDOA 1 (IC 50 = 108.3 µM), hydroxytyrosol acetate 3 (IC 50 = 40.2 µM)and 1-acetoxypinoresinol 4 (IC 50 = 43.8 µM)reduced intracellular Ca 2+ levels after challenging by both A23187 and DNP-BSA, suggesting a mechanism beyond just blockage of extracellular Ca 2+ influx. While luteolin 2, despite its strongest activity (IC 50 = 3.9 µM), reduced Ca 2+ levels only after challenging by A23187, suggesting its main mechanism is by blocking extracellular Ca 2+ influx. We confirmed further, that, each compound decreased the expression of, two or more, calcium channel proteins suggesting that they act as ‘mast cell stabilizers’ by reducing the expression of calcium channel proteins. This work attracts more attention to OMW as a potential source of lead compounds for structural modification to improve and develop new anti-allergic drugs.

元の言語英語
ページ(範囲)207-217
ページ数11
ジャーナルJournal of Functional Foods
58
DOI
出版物ステータス出版済み - 7 1 2019

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Anti-Allergic Agents
Olea
Calcium Channels
calcium channels
inhibitory concentration 50
polyphenols
Calcimycin
Luteolin
Proteins
oleuropein
Mast Cells
proteins
luteolin
Acetates
mast cells
acetates
drugs
Pharmaceutical Preparations
cells

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

これを引用

Anti-allergic activity of polyphenolic compounds isolated from olive mill wastes. / Mwakalukwa, Rogers; Ashour, Ahmed; Amen, Yhiya; Niwa, Yasuharu; Tamrakar, Sonam; Miyamoto, Tomofumi; Shimizu, Kuniyoshi.

:: Journal of Functional Foods, 巻 58, 01.07.2019, p. 207-217.

研究成果: ジャーナルへの寄稿記事

Mwakalukwa, Rogers ; Ashour, Ahmed ; Amen, Yhiya ; Niwa, Yasuharu ; Tamrakar, Sonam ; Miyamoto, Tomofumi ; Shimizu, Kuniyoshi. / Anti-allergic activity of polyphenolic compounds isolated from olive mill wastes. :: Journal of Functional Foods. 2019 ; 巻 58. pp. 207-217.
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