Anti-inflammatory activity and mechanism of action of ethanolic leaf extract of Morinda lucida Benth

Frederick Ayertey, Ebenezer Ofori-Attah, Stephen Antwi, Michael Amoa-Bosompem, Georgina Djameh, Nathaniel Lartey Lartey, Mistuko Ohashi, Kwadwo Asamoah Kusi, Alfred Ampomah Appiah, Regina Appiah-Opong, Laud Kenneth Okine

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

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Background and aim: Most developing countries resort to medicinal plants for treating diseases, but few of these have scientific backing for their use. The aim of the study was to validate traditional use of Morinda lucida leaves in treating inflammation and determine the mechanism of action. Experimental procedure: Effect of hydroethanolic leaf extract of M. lucida (HEML) on localized inflammation was evaluated using rat paw edema presented by sub-planter injections of λ-carrageenan, histamine or serotonin in separate experiments. Systemic inflammation was evaluated by lipopolysaccharide (LPS)-induced hyperthermia. Antioxidant activity of HEML was also evaluated using the free-radical scavenging assay. Results and conclusion: No mortalities were recorded in acute toxicity assay after administering 5000 mg/kg HEML to rats. It showed very good activity against localized and systemic inflammation in inverse dose-dependent manner and caused reduction in nitric oxide and prostaglandin E−2 levels by affecting expression of inducible nitric oxide synthase, but not cyclooxygenases-2 in LPS-activated RAW 264.7 murine macrophages. HEML reduced pro-inflammatory cytokines interleukin (IL)-1β and tumor necrotic factor, but elevated levels of anti-inflammatory cytokine IL-10 in vitro. HEML contains saponins, reducing sugars, polyphenols and flavonoids and showed antioxidant activity with EC50 = 0.6415 ± 0.0027 mg/ml. In conclusion, this study provides evidence that HEML possesses anti-inflammatory activity, possibly through modulation of production of early/late phase inflammation mediators.

本文言語英語
ページ(範囲)249-258
ページ数10
ジャーナルJournal of Traditional and Complementary Medicine
11
3
DOI
出版ステータス出版済み - 5 2021
外部発表はい

All Science Journal Classification (ASJC) codes

  • 補完代替医療

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