Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses FcεRl expression in human basophilic KU812 cells

Yoshinori Fujimura, Hirofumi Tachibana, Mari Maeda-Yamamoto, Toshio Miyase, Mitsuaki Sano, Koji Yamada

研究成果: Contribution to journalArticle査読

95 被引用数 (Scopus)

抄録

We previously found that the O-methylated derivative of (-)-epigallocatechin-3-O-gallate (EGCg), (-)-epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG″3Me), has potent antiallergic activity. The high-affinity IgE receptor, FcεRI, is found at high levels on basophils and mast cells and plays a key role in a series of acute and chronic human allergic reactions. To understand the mechanism of action for the antiallergic EGCG″3Me, the effect of EGCG″3Me on the cell surface expression of FcεRI in human basophilic KU812 cells was examined. Flow cytometric analysis showed that EGCG″3Me was able to decrease the cell surface expression of FcεRI. Moreover, immunoblot analysis revealed that total cellular expression of the FcεRI α chain decreased upon treatment with EGCG″3Me. FcεRI is a tetrameric structure comprising one α chain, one β chain, and two γ chains. The level of mRNA production of each subunit in KU812 cells was investigated. EGCG″3Me reduced FcεRI α and γ mRNA levels. The cross-linkage of FcεRI causes the activation of basophils, which leads to the secretion of inflammatory mediators including histamine. EGCG″3Me treatment inhibited the FcεRI cross-linking-induced histamine release. These results suggested that EGCGε3Me can negatively regulate basophil activation through the suppression of FcεRI expression.

本文言語英語
ページ(範囲)5729-5734
ページ数6
ジャーナルJournal of Agricultural and Food Chemistry
50
20
DOI
出版ステータス出版済み - 9 25 2002

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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