抄録
Hydrogels and their medical applications in tissue engineering have been widely studied due to their three-dimensional network structure, biocompatibility, and cell adhesion. However, the development of an artificial bile duct to replace the recipient’s tissue is still desired. Some challenges remain in the tissue engineering field, such as infection due to residual artifacts. In other words, at present, there are no established technologies for bile duct reconstruction as strength and biocompatibility problems. Therefore, this study investigated hydrogel as an artificial bile duct base material that can replace tissue without any risk of infectious diseases. First, an antibacterial agent (ABA), Finibax (an ABA used for the clinical treatment of biliary tract infection), was immobilized in gelatin using a crosslinking agent, and the antibacterial properties of the gel and its sustainability were tested. Furthermore, the immobilized amount and the improvement of the proliferation of the human umbilical vein endothelial cells (HUVECs) were cultured as the ABA-Gelatin hydrogel was introduced to prepare a 3D scaffold. Finally, we performed hematoxylin and eosin (H&E) staining after subcutaneous implantation in the rat. Overall, the ABA-Gelatin hydrogel was found to be viable for use in hydrogel applications for tissue engineering due to its good bactericidal ability, cell adhesion, and proliferation, as well as having no cytotoxicity to cells.
| 元の言語 | 英語 |
|---|---|
| 記事番号 | 32 |
| ジャーナル | Gels |
| 巻 | 5 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | 出版済み - 6 1 2019 |
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All Science Journal Classification (ASJC) codes
- Organic Chemistry
- Bioengineering
- Biomaterials
- Polymers and Plastics
これを引用
Antibacterial-agent-immobilized gelatin hydrogel as a 3d scaffold for natural and bioengineered tissues. / Iimaa, Tuyajargal; Hirayama, Takaaki; Shirakigawa, Nana; Imai, Daisuke; Yamao, Takanobu; Yamashita, Yo Ichi; Baba, Hideo; Ijima, Hiroyuki.
：: Gels, 巻 5, 番号 2, 32, 01.06.2019.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Antibacterial-agent-immobilized gelatin hydrogel as a 3d scaffold for natural and bioengineered tissues
AU - Iimaa, Tuyajargal
AU - Hirayama, Takaaki
AU - Shirakigawa, Nana
AU - Imai, Daisuke
AU - Yamao, Takanobu
AU - Yamashita, Yo Ichi
AU - Baba, Hideo
AU - Ijima, Hiroyuki
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Hydrogels and their medical applications in tissue engineering have been widely studied due to their three-dimensional network structure, biocompatibility, and cell adhesion. However, the development of an artificial bile duct to replace the recipient’s tissue is still desired. Some challenges remain in the tissue engineering field, such as infection due to residual artifacts. In other words, at present, there are no established technologies for bile duct reconstruction as strength and biocompatibility problems. Therefore, this study investigated hydrogel as an artificial bile duct base material that can replace tissue without any risk of infectious diseases. First, an antibacterial agent (ABA), Finibax (an ABA used for the clinical treatment of biliary tract infection), was immobilized in gelatin using a crosslinking agent, and the antibacterial properties of the gel and its sustainability were tested. Furthermore, the immobilized amount and the improvement of the proliferation of the human umbilical vein endothelial cells (HUVECs) were cultured as the ABA-Gelatin hydrogel was introduced to prepare a 3D scaffold. Finally, we performed hematoxylin and eosin (H&E) staining after subcutaneous implantation in the rat. Overall, the ABA-Gelatin hydrogel was found to be viable for use in hydrogel applications for tissue engineering due to its good bactericidal ability, cell adhesion, and proliferation, as well as having no cytotoxicity to cells.
AB - Hydrogels and their medical applications in tissue engineering have been widely studied due to their three-dimensional network structure, biocompatibility, and cell adhesion. However, the development of an artificial bile duct to replace the recipient’s tissue is still desired. Some challenges remain in the tissue engineering field, such as infection due to residual artifacts. In other words, at present, there are no established technologies for bile duct reconstruction as strength and biocompatibility problems. Therefore, this study investigated hydrogel as an artificial bile duct base material that can replace tissue without any risk of infectious diseases. First, an antibacterial agent (ABA), Finibax (an ABA used for the clinical treatment of biliary tract infection), was immobilized in gelatin using a crosslinking agent, and the antibacterial properties of the gel and its sustainability were tested. Furthermore, the immobilized amount and the improvement of the proliferation of the human umbilical vein endothelial cells (HUVECs) were cultured as the ABA-Gelatin hydrogel was introduced to prepare a 3D scaffold. Finally, we performed hematoxylin and eosin (H&E) staining after subcutaneous implantation in the rat. Overall, the ABA-Gelatin hydrogel was found to be viable for use in hydrogel applications for tissue engineering due to its good bactericidal ability, cell adhesion, and proliferation, as well as having no cytotoxicity to cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=85068362925&partnerID=8YFLogxK
U2 - 10.3390/gels5020032
DO - 10.3390/gels5020032
M3 - Article
VL - 5
JO - Gels
JF - Gels
SN - 2310-2861
IS - 2
M1 - 32
ER -