Antibacterial effects of glycyrrhetinic acid and its derivatives on Staphylococcus aureus

Kentaro Oyama, Miki Kawada-Matsuo, Yuichi Oogai, Tetsuya Hayashi, Norifumi Nakamura, Hitoshi Komatsuzawa

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Staphylococcus aureus is a major pathogen in humans and causes serious problems due to antibiotic resistance. We investigated the antimicrobial effect of glycyrrhetinic acid (GRA) and its derivatives against 50 clinical S. aureus strains, including 18 methicillin-resistant strains. The minimum inhibitory concentrations (MICs) of GRA, dipotassium glycyrrhizate, disodium succinoyl glycyrrhetinate (GR-SU), stearyl glycyrrhetinate and glycyrrhetinyl stearate were evaluated against various S. aureus strains. Additionally, we investigated the bactericidal effects of GRA and GR-SU against two specific S. aureus strains. DNA microarray analysis was also performed to clarify the mechanism underlying the antibacterial activity of GR-SU. We detected the antimicrobial activities of five agents against S. aureus strains. GRA and GR-SU showed strong antibacterial activities compared to the other three agents tested. At a higher concentration (above 2x MIC), GRA and GR-SU showed bactericidal activity, whereas at a concentration of 1x MIC, they showed a bacteriostatic effect. Additionally, GRA and GRSU exhibited a synergistic effect with gentamicin. The expression of a large number of genes (including transporters) and metabolic factors (carbohydrates and amino acids) was altered by the addition of GR-SU, suggesting that the inhibition of these metabolic processes may influence the degree of the requirement for carbohydrates or amino acids. In fact, the requirement for carbohydrates or amino acids was increased in the presence of either GRA or GRSU. GRA and GR-SU exhibited strong antibacterial activity against several S. aureus strains, including MRSA. This activity may be partly due to the inhibition of several pathways involved in carbohydrate and amino acid metabolism.

元の言語英語
記事番号e0165831
ジャーナルPloS one
11
発行部数11
DOI
出版物ステータス出版済み - 11 1 2016

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Glycyrrhetinic Acid
Staphylococcus aureus
chemical derivatives
Derivatives
acids
Microbial Sensitivity Tests
minimum inhibitory concentration
Carbohydrates
carbohydrates
Amino Acids
amino acids
Stearates
Methicillin Resistance
Methicillin
antibacterial properties
amino acid metabolism
methicillin
Pathogens
anti-infective properties
gentamicin

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Oyama, K., Kawada-Matsuo, M., Oogai, Y., Hayashi, T., Nakamura, N., & Komatsuzawa, H. (2016). Antibacterial effects of glycyrrhetinic acid and its derivatives on Staphylococcus aureus. PloS one, 11(11), [e0165831]. https://doi.org/10.1371/journal.pone.0165831

Antibacterial effects of glycyrrhetinic acid and its derivatives on Staphylococcus aureus. / Oyama, Kentaro; Kawada-Matsuo, Miki; Oogai, Yuichi; Hayashi, Tetsuya; Nakamura, Norifumi; Komatsuzawa, Hitoshi.

:: PloS one, 巻 11, 番号 11, e0165831, 01.11.2016.

研究成果: ジャーナルへの寄稿記事

Oyama, K, Kawada-Matsuo, M, Oogai, Y, Hayashi, T, Nakamura, N & Komatsuzawa, H 2016, 'Antibacterial effects of glycyrrhetinic acid and its derivatives on Staphylococcus aureus', PloS one, 巻. 11, 番号 11, e0165831. https://doi.org/10.1371/journal.pone.0165831
Oyama, Kentaro ; Kawada-Matsuo, Miki ; Oogai, Yuichi ; Hayashi, Tetsuya ; Nakamura, Norifumi ; Komatsuzawa, Hitoshi. / Antibacterial effects of glycyrrhetinic acid and its derivatives on Staphylococcus aureus. :: PloS one. 2016 ; 巻 11, 番号 11.
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