Antibiotic effects against periodontal bacteria in organ cultured tissue

Masaaki Takeshita, Akira Haraguchi, Mayumi Miura, Takafumi Hamachi, Takao Fukuda, Terukazu Sanui, Aiko Takano, Fusanori Nishimura

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

Mechanical reduction of infectious bacteria by using physical instruments is considered the principal therapeutic strategy for periodontal disease; addition of antibiotics is adjunctive. However, local antibiotic treatment, combined with conventional mechanical debridement, has recently been shown to be more effective in periodontitis subjects with type 2 diabetes. This suggests that some bacteria may invade the inflamed inner gingival epithelium, and mechanical debridement alone will be unable to reduce these bacteria completely. Therefore, we tried to establish infected organ culture models that mimic the inner gingival epithelium and aimed to see the effects of antibiotics in these established models. Mouse dorsal skin epithelia were isolated, and periodontal bacteria were injected into the epithelia. Infected epithelia were incubated with test antibiotics, and colony-forming ability was evaluated. Results indicated that effective antibiotics differed according to injected bacteria and the bacterial combinations tested. Overall, in organ culture model, the combination of amoxicillin or cefdinir and metronidazole compensate for the effects of less effective bacterial combinations on each other. This in vitro study would suggest effective periodontal treatment regimens, especially for severe periodontitis.

本文言語英語
ページ(範囲)5-12
ページ数8
ジャーナルClinical and Experimental Dental Research
3
1
DOI
出版ステータス出版済み - 2 1 2017

All Science Journal Classification (ASJC) codes

  • 歯科学(全般)

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