Antimicrobial activity of culture filtrate of Bacillus amyloliquefaciens RC-2 isolated from mulberry leaves

S. Yoshida, Syuntaro Hiradate, T. Tsukamoto, K. Hatakeda, A. Shirata

研究成果: ジャーナルへの寄稿記事

167 引用 (Scopus)

抄録

A potential antagonist, Bacillus amyloliquefaciens strain RC-2, against Colletotrichum dematium, mulberry anthracnose fungus, was obtained from healthy mulberry leaves by in vitro and in vivo screening techniques. Application of culture filtrate of RC-2 inhibited disease on mulberry leaves, indicating that suppression was due to antifungal compounds in the filtrate. Development of mulberry anthracnose on mulberry leaves was inhibited only when the culture filtrate was applied before fungal inoculation, and it was not inhibited by application after inoculation. These results suggest that the antifungal compounds in the filtrate exhibit a preventive effect on the disease. Peptone significantly increased production of the antifungal compounds. The culture filtrate of RC-2 also inhibited the growth of several other phytopathogenic fungi and bacteria, such as Rosellinia necatrix, Pyricularia oryzae, Agrobacterium tumefaciens, and Xanthomonas campestris pv. campestris, in vitro. From the culture filtrate of RC-2, seven kinds of antifungal compounds were isolated by high performance liquid chromatography analysis, and one of the compounds was determined as iturin A2, a cyclic peptide, by nuclear magnetic resonance and fast atom bombardment mass analysis.

元の言語英語
ページ(範囲)181-187
ページ数7
ジャーナルPhytopathology
91
発行部数2
DOI
出版物ステータス出版済み - 1 1 2001
外部発表Yes

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Bacillus amyloliquefaciens
culture filtrates
mulberries
anti-infective agents
filtrates
anthracnose
leaves
Rosellinia necatrix
Colletotrichum dematium
Xanthomonas campestris pv. campestris
cyclic peptides
Magnaporthe oryzae
plant pathogenic bacteria
plant pathogenic fungi
peptones
Agrobacterium radiobacter
nuclear magnetic resonance spectroscopy
antagonists
high performance liquid chromatography
screening

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

これを引用

Antimicrobial activity of culture filtrate of Bacillus amyloliquefaciens RC-2 isolated from mulberry leaves. / Yoshida, S.; Hiradate, Syuntaro; Tsukamoto, T.; Hatakeda, K.; Shirata, A.

:: Phytopathology, 巻 91, 番号 2, 01.01.2001, p. 181-187.

研究成果: ジャーナルへの寄稿記事

Yoshida, S. ; Hiradate, Syuntaro ; Tsukamoto, T. ; Hatakeda, K. ; Shirata, A. / Antimicrobial activity of culture filtrate of Bacillus amyloliquefaciens RC-2 isolated from mulberry leaves. :: Phytopathology. 2001 ; 巻 91, 番号 2. pp. 181-187.
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