This study aimed to develop an antimicrobial biodegradable food packaging for control of pathogens in pangasius fish fillets, one of the world's most consumed freshwater fish. Bacteriocin 7293 (Bac7293), a novel bacteriocin from Weissella hellenica BCC 7293, was diffusion coated onto poly (lactic acid)/sawdust particle biocomposite film (PLA/SP) with the maximum amount of 19.54 ± 2.87 μg/cm2. According to the JIS Z 2801:2000 standard testing method, the produced PLA/SP film impregnated with Bac7293 (PLA/SP + Bac7293) effectively inhibited both Gram-positive (Listeria monocytogenes and Staphylococcus aureus) and Gram-negative bacteria (Pseudomonas aeruginosa, Aeromonas hydrophila, Escherichia coli and Salmonella Typhimurium) which have been considered as a reason for the rejection of pangasius fish fillets. This antimicrobial film inhibited the growth of target microorganisms by about 2–5 log CFU/cm2, depended on bacterial strains, compared with control, both in vitro and in pangasius fish fillets by means of challenge test. The overall migration of PLA/SP + Bac7293 film to food simulants was maximized in isooctane (3.67 ± 0.47 mg/dm2) which was much lower than the overall migration limit regulated by the commission of the European communities. This suggested high potential application of PLA/SP + Bac7293 as a good antimicrobial packaging for pangasius fish fillets.
All Science Journal Classification (ASJC) codes
- Food Science